2002年3月29日 · Dominant negative forms of Ras and Raf abolished the activation of VEGF promoter by acidic pH. These results show that acidic pH activates Ras and the ERK1/2 MAPK pathway to enhance VEGF transcription via AP-1, leading to increased VEGF production.

In vitro, acidic pH considerably reduced VEGF-mediated endothelial cell responses, suggesting that in acidic conditions endothelial cells prosper independently of VEGF. Accordingly, we found that low pH values significantly reduced the expression of VEGFR-2 by EC.

2003年5月23日 · pH Alters the Affinity of VEGF 165 and VEGF 121 for Heparin—The increased binding of VEGF to endothelial cells and the extracellular matrix at low pH suggest that VEGF interactions with heparan sulfate are enhanced under acidic conditions.

2002年3月29日 · Overexpression of vascular endothelial growth factor (VEGF) is associated with disease progression in human glioblastomas. We recently showed that VEGF promoter activity is inversely correlated with tumor extracellular pH (pH o) …

2004年1月16日 · Vascular endothelial growth factor (VEGF) binding to endothelial cells and the extracellular matrix (ECM) increases at acidic pH (7.0-5.5). These interactions are dependent on heparan sulfate proteoglycans, but do not depend on the presence of VEGF receptors.

2001年7月5日 · In summary, we found that transient exposure to a low extracellular pH (6.7–7.1) induced an increased steady-state level of VEGF mRNA expression, which correlated with prolonged VEGF mRNa ...

2003年5月23日 · Thus, the present study investigates the consequences of acidic pH on VEGF binding and activity in endothelial cell cultures. We found that the binding of VEGF165 and VEGF121 to endothelial cells increased as the extracellular pH was decreased from 7.5 to 5.5.

Physical interactions between vascular endothelial growth factor (VEGF), a central player in blood endothelial cell biology, and fibronectin, a major fibrillar protein of the extracellular matrix, are important determinants of angiogenic activity in health and disease.

Here we show that substitution of the neutral culture medium (pH 7.3) with acidic pH medium (pH 6.6) up-regulates VEGF mRNA and protein production in human glioblastoma cells as reflected by Northern blot analysis and enzyme-linked immunosorbent assay.

血管内皮生长因子（vascular endothelial growth factor，VEGF），又称血管通透因子（vascular permeability factor，VPF）是一种高度特异性的促血管内皮细胞生长因子，具有促进血管通透性增加、细胞外基质变性、血管内皮细胞迁移、增殖和血管形成等作用。