2022年2月21日 · Using two different approaches to introduce 8-oxoG in cells, we show that DDB2 is recruited to 8-oxoG immediately after damage and colocalizes with 8-oxoG glycosylase (OGG1) at sites of repair....

2019年7月22日 · Single-molecule real-time imaging revealed that UV-DDB forms transient complexes with OGG1 or APE1, facilitating their dissociation from DNA. Furthermore, UV-DDB moves to sites of 8-oxoG...

This review first discusses biochemical studies demonstrating how UV-DDB stimulates human 8-oxoG glycosylase (OGG1), MUTYH, and apurinic/apyrimidinic (AP) endonuclease (APE1) to increase their turnover at damage sites.

In mammalian cells, the modified base is excised by the 8-oxoguanine DNA glycosylase (OGG1), initiating the base excision repair (BER) pathway. OGG1 confronts the massive challenge that is finding rare occurrences of 8-oxoG among a million-fold excess of normal guanines.

Recombinant Human N-Glycosylase/OGG1 是一种DNA修复酶，其作为一种修饰酶, 主要具有DNA糖基化酶活性和脱嘌呤/脱嘧啶（apurinic/apyrimidinic，AP）裂解酶活性两种活性。 N-糖基化酶活性能从双链DNA 上去除受损嘌呤碱基产生脱嘌呤 (AP) 位点，而 AP 裂解酶活性则能从 AP 位点的 3 处进行切割产生一个 5 磷酸和一个 3 磷酸-α，β-不饱和醛。 此外OGG1还能切割其它异常碱基对，比如与胞嘧啶配对的7,8-二羟基-8-氧代鸟嘌呤 (8-氧代鸟嘌呤), 与胞嘧啶配对的8-羟基 …

2023年6月15日 · Moreover, we have shown that UV-DDB can assist chromatin decompaction, facilitating access of OGG1 to 8-oxoguanine damage in telomeres. This review summarizes the biochemistry, single-molecule, and cell biology approaches that our group used to directly demonstrate the essential role of UV-DDB in BER.

4 天之前 · The first step of the process is cleavage of the 8-oxoguanine moiety by the glycosylase OGG1, yielding an abasic site in the genome [11, 12]. Repair of the abasic site proceeds initially with the function of the human ... UV spectroscopy. Concentration of oligonucleotides was determined at a wavelength of 260 nm on a Cary 3500 UV spectrometer ...

Fold change in enzymatic activity of OGG1, APE1, Pol β, MUTYH, AAG/MPG, and SMUG1 in the presence of UV-DDB. Base excision repair (BER) is a cellular process that removes damaged bases arising...

Single-molecule real-time imaging revealed that UV-DDB forms transient complexes with OGG1 or APE1, facilitating their dissociation from DNA. Furthermore, UV-DDB moves to sites of 8-oxoG repair in cells, and UV-DDB depletion sensitizes cells to oxidative DNA damage.

UV-DDB is well known for recognizing UV photoproducts and initiating the global genome nucleotide excision repair pathway. The discovery that UV-DDB binds to 8-oxoG lesions and abasic sites, led to the novel finding that UV-DDB enhances OGG1-mediated excision of 8-oxoG, facilitating OGG1 enzymatic turnover by displacing it from the abasic site ...