A transposase is any of a class of enzymes capable of binding to the end of a transposon and catalysing its movement to another part of a genome, typically by a cut-and-paste mechanism or a replicative mechanism, in a process known as transposition.

执行转座功能的酶，通常由转座子编码，识别 转座子 两端的特异序列，能把转座子从相邻序列中脱离出来， 再插入 到新的DNA靶位点，无 同源性 要求。 TnsABC*转座酶可用在 GPS TM系列系统中（GPS-1 基因组测序系统，GPS-M突变系统和GPS-LS蛋白质结构分析系统）将pGPS质粒中的Transprimer转座元件体外随机插入到任何期望的靶DNA内 (1-3)。 三个组成蛋白分别从含有编码TnsA、TnsB和TnsC*的质粒的E.coliK-12中纯化获得。 用于 GPS系统 中所用到的Tn7源转座 …

Transposases are enzymes involved in the cutting and splicing of large quantities of DNA. Transposons, themselves, are segments of DNA that are able to move to different positions in the genome of the same cell. Transposons have been referred to as “jumping genes” which can create duplicate genes or mutations.

DNA transposases are enyzmes that move discrete segments of DNA called transposons from one location in the genome (often called the donor site) to a new site without using RNA intermediates. DNA transposases are usually encoded by the mobile element itself (in which case they are “autonomous” transposons).

In this review, we discuss what is known about the different ways in which DNA transposons are mobilized by their associated transposase enzymes. These biochemical pathways are placed in the context of the structural information available for DNA transposases and the complexes they form with DNA (“transpososomes”).

Tn5 Transposase是野生型Tn5转座酶的突变形式。Tn5转座酶是Tn5转座子的核心酶，具有高效的切割和插入活性。Tn5转座酶识别其转座子序列的内端(inside end, IE)、外端(outside end, OE)和嵌合端(mosaic end, ME)序列。

The enzyme shown here is a bacterial transposase (PDB entry 1muh ) that moves a transposon called Tn5. The Tn5 transposon is a piece of DNA that includes several genes for antibiotic resistance, along with the gene needed to build the transposase itself.

Transposase enzymes are divided into several families based on the mechanism utilized during transposition — DD[E/D]-transposases, rolling-circle or Y2-transposases, tyrosine (Y)-transposases, serine (S)-transposases, and a family, which encodes a combination of reverse transcriptase and endonuclease activities (RT/En) [14]. Members of the DD ...

2024年6月26日 · Using IS 21 as a model transposase system, we show how an ATPase regulator uses nucleotide-controlled assembly and DNA deformation to enable structure-based site selectivity, transposase...

2010年3月9日 · Transposase genes encode DNA-binding enzymes, members of the polynucleotidyl transferase superfamily, that catalyze ‘cut-and-paste’ or ‘copy-and-paste’ reactions promoting the movement of DNA segments to new sites ( 80). The term transposase is often used to describe what are classically known as DNA transposases or type II transposases.