2024年4月23日 · 该研究整合了烟草花叶病毒TMV-GFP载体和农杆菌介导的瞬时表达技术，利用模式植物本氏烟(Nicotiana benthamiana)，开发了一种高效的植物病原菌效应子筛选系统。

2020年5月21日 · 基础质粒p4GD-PL包含设计好的TMV基因组，将PCR扩增的0.7kb的GFP-ORF构建在基础质粒的MCS区域，产生了构建好的载体，并命名为p4GD-GFP。 示意图如图： 利用p4GD-GFP体外转录的侵染性4GD-GFP转录本侵染烟草叶片，大约24h后可以在荧光显微镜下观察到荧光。 接种点在40h观察时是很亮的1mm直径的圆，以1mm/d的速度增加。 当扩散区域碰到叶脉后会快速沿着叶脉传播，2mm/d。 然而在韧皮部（phloem）或木质部（xylem）感染的组 …

In this study, we designed a novel screening system that combines the TMV (tobacco mosaic virus)-GFP vector and Agrobacterium-mediated transient expression in the model plant Nicotiana benthamiana. This system enables the rapid identification of effectors that …

2024年4月22日 · In this study, we designed a novel screening system that combines the TMV (tobacco mosaic virus)-GFP vector and Agrobacterium -mediated transient expression in the model plant Nicotiana benthamiana. This system enables the rapid identification of effectors that interfere with plant immunity.

2023年12月7日 · Based on the aforementioned results, the effect of TMV PQS5 on TMV genome proliferation in plant was evaluated by constructing a transient expression vector of TMV–green fluorescent protein (GFP) , where fluorescence signals visually reflected TMV genome replication .

2022年9月1日 · Tobacco mosaic virus (TMV) seriously affects the yield and quality of tobacco leaves. The expression of TMV in tobacco leaves can be analyzed by detecting green fluorescence-related traits after inoculation with the infectious clone of TMV-GFP (Tobacco mosaic virus - green fluorescent protein).

Chrysanthemums (Chrysanthemum morifolium) are susceptible to tobacco mosaic virus (TMV). TMV-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (GFP) to allow visualization of TMV movement.

Chrysanthemums (Chrysanthemum morifolium) are susceptible to tobacco mosaic virus (TMV). TMV-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (GFP) to allow visualization of TMV movement.

接种tmv-gfp（烟草花叶病毒-绿色荧光蛋白）的感染性克隆后，可通过检测绿色荧光相关性状来分析烟草叶片中tmv的表达。 然而，传统的检测 TMV-GFP 的方法既费时又费力，而且大多需要大量的手动程序。

在这项研究中，我们设计了一种新型筛选系统，将TMV（烟草花叶病毒）-GFP载体与农杆菌介导的瞬时表达结合在模型植物本塞姆氏烟草中。 该系统能够快速识别干扰植物免疫力的效应子。 通过在短短几天内使用紫外灯观察 GFP 荧光信号，可以轻松评估这些效应器的生物学功能。 为了评估 TMV-GFP 系统，我们最初使用来自细菌病原体Ralstonia solanacearum的毒力和无毒力 III 型效应子对其进行了测试。 在证明了TMV-GFP系统的准确性和效率后，我们利用这种方法成功筛 …