2022年2月20日 · Comprehensive WB procedure for cell culture and tissue samples with chemiluminescent and fluorescent detection. Western blotting is a technique that uses specific antibodies to identify proteins separated by size through gel electrophoresis. The immunoassay uses a membrane made of nitrocellulose or PVDF (polyvinylidene fluoride).

2025年2月15日 · Western Blot 是一种基于凝胶电泳和抗体特异性识别的分子生物学技术。 它利用 SDS-PAGE 凝胶电泳分离样品中的蛋白质，然后将蛋白质转移到膜上（PVDF 或 硝酸纤维素膜）。 通过抗体与目标蛋白的特异性结合，以及酶或荧光标记的二抗对一抗的识别，最后通过 化学发光 、荧光或显色等方法检测目标蛋白的表达情况。 裂解缓冲液 (Lysis Buffer): (根据需要选择，例如 RIPA buffer, NP-40 buffer)。 常用的RIPA buffer 配方 (可根据需要调整成分):

This protocol outlines how to extract protein from mouse tissues and running a Western Blot. In brief:

Western blot 是一项通过凝胶电泳按照分子量大小分离蛋白，然后再利用 特异性抗体 识别这些蛋白的技术。 免疫检测通常使用由硝酸纤维素或 PVDF（聚偏二氟乙烯）制成的膜。 将凝胶紧贴膜放置，蛋白在电流作用下从凝胶迁移到膜上。 然后利用目标靶点特异性抗体对膜做进一步处理，再通过二抗和检测试剂让膜显色。 查看下方的 western blot 实验方案视频。 查看更多实验方案视频，请 单击访问 我们的方案视频库。 >> 打印完整的 western blot 实验方案. >> 查看我们的 …

Tips and tricks for producing high quality western blots of tissue lysates. Including lysis buffer choice, protein quantification and reducing high background from endogenous immunoglobulins using TidyBlot.

Selecting Loading Controls for Mouse Tissue Western Blots. How does a Cell Signaling Technology (CST) scientist prepare for testing mouse tissues by western blot? We check reliable expression databases and the literature to ensure the tissue type expresses a loading control protein before doing a western blot.

Good sample preparation is critical for successful separation of protein bands in electrophoresis and western blot detection. No single sample preparation method or buffer will work for all sample types due to the diversity of protein samples.

Here we cover all essential details of sample preparation for western blot, including obtaining lysate from cell culture and tissues, measuring protein concentration, and reducing and denaturing your sample. Before setting up a western blot experiment, inform yourself about the characteristics of the protein of interest. Those include:

All the steps for protein extraction from cells or tissue (fresh or frozen) must be performed at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein samples. Visit the Calculators page for a list of recipes …

wb检测样本前处理：细胞裂解和蛋白提取 从细胞或组织（新鲜或冷冻）中提取蛋白质的所有步骤必须在2-8°C下进行。 以下是用于制备蛋白质样品的一种常见裂解缓冲液的成分。