2N4R/ Tau-441 是Tau全长蛋白 ， K18 和K19 是截短的Tau蛋白，仅含有微管结合重复区域。 Tau蛋白 的微管结合能力由翻译后修饰来控制，其中磷酸化修饰是主要方式之一。

2017年11月22日 · Here, we investigate the sequence determinants and consequences of the interactions of the longest Tau isoform (2N4R), the K18 fragment, a C-terminal Tau fragment containing its four...

To provide more insight about how lipids can modulate the interactions with Tau, we have conducted a comprehensive Atomic Force Microscopy (AFM) study involving supported lipid bilayers of controlled compositions with the Tau microtubule-binding construct K18.

2022年3月22日 · 生物物理研究发现了K18凝聚中的热点，并揭示了该片段的LLPS与重复序列的KXGS基序内的残基从延伸构象到瞬时β发夹状状态的转变有关，而另一项研究表明该分子总体上是扩张的。 尽管它们很有趣，但尚不清楚从非生理性K18片段的研究中得出的教训是否适用于全 …

2024年3月19日 · K18 is the microtubule-binding domain of Tau protein and plays a vital role in the microtubule assembly, recycling processes, and amyloid fibril formation. Here, we efficiently explore the conformation of K18 with about 150 μs lifetimes in silico .

The Tau K18 fragment comprises all four microtubule binding repeats (4R) of Tau with mutations C291S and C322S. The K18 fragment of Tau has a higher propensity of aggregation than the full-length protein 1, with this mutant form serving as a non-aggregating control 2.

2015年4月30日 · Here we use single-molecule fluorescence to study the aggregation of the repeat domain of tau (K18), and two mutant forms linked with familial frontotemporal dementia, the deletion mutant ΔK280...

2024年12月5日 · The most widely used tau biosensor cell line expresses the K18 aggregation-prone tau fragment with aggregation-inducing mutations P301L and V337M, fused with CFP or YFP at the C terminus. 7 Tau K18 biosensor cells have been used to detect seeding-competent tissues, 8, 9 fibrils, 6, 7 and oligomers 10 and to aid in inhibitor discovery. 11, 12, 13

2013年4月28日 · To quantify tau acetyltransferase activity, we performed filter-binding assays using recombinant wild-type tau-K18 or cysteine mutants (tau-K18-2CA and tau-K18-2CS) containing either...

全原子分子动力学模拟显示，Δk280 显著提高了 k18 单体的塌陷度和 β 片含量，表明 Δk280 突变可能促进 k18 llps，通过粗粒相共存模拟和显微镜实验验证。