TT-seq maps the human transient transcriptome | Science - AAAS
2016年6月3日 · We developed transient transcriptome sequencing (TT-seq), a protocol that uniformly maps the entire range of RNA-producing units and estimates rates of RNA synthesis …
TT-seq | Nascent Transcriptomics Core - Harvard University
TT-seq is a variant of 4-thiouridine (4sU) sequencing. This approach labels newly synthesized RNA by metabolic incorporation of 4sU in live cells. Analysis of this RNA can provide …
TT-seq文献阅读(TT-seq maps the human transient transcriptome)
2020年6月23日 · 作者开发了瞬时转录本测序（TT-seq），这个方法绘制转录的活性区域，可以估测RNA合成和降解速率。TT-seq是基于4sU-seq的一项技术，将细胞暴露在核苷类似物4-硫尿 …
Using TT chem -seq for profiling nascent transcription and
2020年1月8日 · Here, we describe a detailed protocol for TT chem -seq that enables obtaining high-resolution transcriptome profiles of nascent and newly transcribed RNA by using …
TT-seq captures enhancer landscapes immediately after T-cell ...
2017年3月7日 · TT-seq maps eRNAs and mRNAs every 5 min after T-cell stimulation with high sensitivity and identifies many new primary response genes. TT-seq reveals that the synthesis …
Science：开发出TT-Seq技术绘制人类瞬时转录组图谱
” Gagneur补充道，“利用TT-Seq方法，我们如今拥有一种合适的工具来更多地了解基因在不同类型细胞中如何受到控制和基因调节程序如何工作。 在很多情形下，研究人员对哪些基因在某种 …
transient transcriptome sequencing (TT-seq) , a protocol that uniformly maps the entire range of RNA-producing units and estimates rates of R NA synthesis and degradation. Application of …
26 experimental protocol for transient transcriptome sequencing (TT-seq). TT-seq detects 27 metabolically labeled, newly synthesized RNA fragments genome-wide in living cells.
TT-seq maps the human transient transcriptome | RNA-Seq Blog
Application of TT-seq to human K562 cells recovers stable messenger RNAs and long intergenic noncoding RNAs and additionally maps transient enhancer, antisense, and promoter …
TT-seq maps the human transient transcriptome - PubMed
2016年6月3日 · We developed transient transcriptome sequencing (TT-seq), a protocol that uniformly maps the entire range of RNA-producing units and estimates rates of RNA synthesis …