Tn10 is a transposable element, which is a sequence of DNA that is capable of mediating its own movement from one position in the DNA of the host organism to another. There are a number of different transposition mechanisms in nature, but Tn 10 uses the non-replicative cut-and-paste mechanism. [1] .

Tn10转位子是依靠同源性而能移动的DNA节段即称为转位因子。 Tn10两端的IS（右侧IS10R，左侧为IS10L）即不完全相同。 在IS10两端的反向重复顺序长22bp。 这个22bp的反向重复即是转位酶所赖以识别的位点，因而为转位反应所必须。 由于IS10R和IS10L的反向重复不完全相同，所以IS10R是Tn10转位所必须，而IS10L的转位活性却仅为IS10R的1-10%。 细菌体内编码抗药性（例如抗四环素和青霉素）的基因存在于质粒中。 质粒与 细菌染色体 之间几乎没有什么同源性。 …

Transposon Tn10 is a composite element in which two individual insertion sequence (IS)-like sequences cooperate to mediate transposition of the intervening material. The two flanking IS10 elements are not identical; IS10-right is responsible for functions required to promote transposition, and IS10- …

2020年11月23日 · Tn10 的两端是两个取向相反的IS1O，中间有抗四环素的抗性基因 (TetR)，当Tnl0整合在一个环状DNA分子中间时，就可以产生新的转座子。 当转座子转座插入宿主DNA时，在插入处产生正向重复序列，其过程是这样的:先是在靶DNA插入处产生交错的切口，使靶DNA产生两个突出的单链末端，然后转座子同单链连接，留下的缺口补平，最后就在转座子插入处生成了宿主DNA的正向重复。 转座子是一类在细菌的染色体，质粒或噬菌体之间自行移动的遗传成 …

The study of the bacterial transposons Tn10 and Tn5 has provided a wealth of information regarding steps in nonreplicative DNA transposition, transpososome dynamics and structure, as well as mechanisms employed to regulate transposition.

Tn10 is a composite transposon. It comprises a pair of IS10 insertion sequences located in opposite orientation flanking ~6.7 kb of unique sequences; these unique sequences encode a tetracycline resistance determinant and other determinants whose functions remain to...

2015年1月29日 · Tn 10 and Tn 5 are composite bacterial transposons (Fig. 1). Both these transposons, as well as their respective IS elements (IS 10 and IS 50), transpose by a nonreplicative cut-and-paste mechanism.

A Tn10 transposon was used to insert a T7 RNA polymerase promoter at random locations throughout the genome of approximately 10 5 E. coli cells. The resultant library was comprised of individual variants defective in at least one gene in which the inserted sequence was located.

2023年12月22日 · 转座酶在转座过程中发挥着关键作用，一些转座子，比如Tn10，可以通过控制转座酶的合成调控转座发生的频率。 限制转座子转座频率的第二个途径是把转座过程局限在细胞周期的某一特定阶段。

2009年3月12日 · Tn10转座子和IS10究竟是什么关系？Tn10转座子可以自发转座，但是由于其转录速率低导致其转座率极低。宿主基因组或质粒表达的转座酶可提高其专座率。 IS10也可自发转座，而且值得指出的是，Tn10的两端就是S10。Tn10的