3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). [1] . TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate.

3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.

The rate constant for compound II reduction to native enzyme by TMB obtained under steady-state conditions is (9.4 +/- 0.6) x 10(5) M-1 s-1. The results are applied to a new, more accurate assay for myeloperoxidase based upon the formation of the charge-transfer complex between TMB and its diimine final product.

TMB is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB can function as a photoacoustic probe for reactive oxygen and nitrogen species ( ROS/RNS ), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to ...

2020年11月4日 · 自从3,3',5,5'-四甲基联苯胺（tmb）首次在基于hrp的免疫分析中应用以来，tmb是迄今为止最常用的生色底物，其显色反应基于h2o2存在下hrp催化氧化tmb。 在TMB出现之后，逐渐取代了其他联苯胺衍生物在比色测定中的使用。

tmb已在逐步取代强致癌物联苯胺和其他致癌性的联苯胺衍生物，应用于临床化验、法医检验、刑事侦破及环境监测等领域；尤其是在临床生化检验方面，tmb作为过氧化酶的新底物，在酶免疫分析法（eia）和酶联免疫吸附检验法（elisa）中获得了广泛的应用。

Tetramethylbenzidine (TMB) is a non-toxic and non-mutagenic colorless dye that develops a distinctive blue color upon oxidation. In this work, we have investigated the potential of TMB as an acoustogenic photoacoustic probe for ROS/RNS.

赛默飞提供高质量的tmb发色剂溶液，专为elisa实验设计，确保实验结果的准确性和可靠性。 我们的发色剂溶液具有稳定性高、灵敏度强等特点，适用于各种ELISA应用。

tmb（3,3′,5,5′-四甲基联苯胺）是一种用于免疫组织化学染色程序的生色底物，同时也是酶联免疫吸附测定（elisa）的可视化试剂。 立即从AbMole中国订购高品质Fluorescent Dye抑制剂TMB！

TMB (3,3′, 5,5′ tetramethylbenzidine dihydrochloride) is a sensitive chromogen substrate for peroxidase and a non-carcinogenic substitute for benzidine. TMB can be used for blotting, immunohistochemical, and in situ hybridization applications.