A DNA recombination-based approach to eliminate papillomavirus ...
2005年3月9日 · In this study, we have developed a DNA recombination-based approach for the conditional expression of the suicide HSV-1 thymidine kinase (TK) gene. The gene cassette is …
The truncated C-terminal E2 (E2-TR) protein of bovine …
2012年8月1日 · Surprisingly, the E2-TR protein could consistently activate transcription in a variety of assays from a minimal tk promoter as well as the BPV-1 major early promoter (P89), …
Recovery of a hybrid vector, derived from bovine papilloma
A bovine papilloma virus (BPV)-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase (tk) gene is capable of transforming …
Recovery of a hybrid vector derived from bovine papilloma virus …
1983年3月1日 · A bovine papilloma virus (BPV)-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase ( tk) gene is …
The tumour promoter TPA enhances the transformation to the …
1986年1月1日 · The tumour promoter TPA (12-O-tetradecanoylphorbol-13-acetate) increased the transformation of LATK − mouse cells to the TK + phenotype when the cells were transfected …
The functional BPV-1 E2 trans-activating protein can act as a …
E2 stimulated transcription of the HSV thymidine kinase (TK) promoter when E2-binding sites were positioned in an enhancer configuration approximately 100 bp upstream of the promoter …
Recovery of a hybrid vector derived from bovine papilloma virus …
A bovine papilloma virus (BPV)-pBR322-derived recombinant plasmid carrying a 2-kb fragment of Herpes simplex virus DNA containing the thymidine kinase (tk) gene is capable of transforming …
Bovine papilloma virus contains an activator of gene expression at …
Bovine papilloma virus (BPV) contains a cis-acting DNA element which can enhance transcription of distal promoters. Utilizing both direct and indirect transient transfection assays, we showed …
The BPV-1 E2 protein does not efficiently activate a
BPV-1 E2 protein was supplied by co-transfecting pC59 and RSV3-GAL was used as a control for transfection efficiency. Known tk promoter elements are indicated.
The lcr/tk hybrid promoter retains the enhanced epithelial …
Previously, we have demonstrated that the BPV-4 promoter shows an enhanced epithelial response to activation, not only by E2 but also by VP16-E2 and VP16-LexA (39).