CAR-T细胞载体拷贝数检测的qPCR法考量 - 翌圣生物
tcr-t细胞疗法的作用机制是先从患者血液或肿瘤组织中分离出t细胞，然后从单个t细胞克隆中分离出tcrα和β链，并插入慢病毒或逆转录病毒载体中，再进行病毒转染t细胞，将目标tcrαβ序列引入t …
Flow cytometry and IG/TCR quantitative PCR for minimal ... - Nature
2012年8月16日 · Minimal residual disease (MRD) quantification is widely used for therapeutic stratification in pediatric acute lymphoblastic leukemia (ALL). A robust, reproducible, sensitivity …
Frontiers | Quantitative Characterization of the T Cell Receptor ...
2017年10月11日 · We used a constant region qPCR to measure the number of TCR molecules per T cell in six samples of PBMC from healthy volunteers (Figure S4 in Supplementary …
Diagnostic role of tests for T cell receptor (TCR) genes
Rapid advances in molecular biological techniques have made it possible to study disease pathogenesis at a genomic level. T cell receptor (TCR) gene rearrangement is an important …
An adjusted droplet digital PCR assay for quantification of vector …
2024年12月4日 · In this study, we developed a robust assay for assessing the VCN of chimeric antigen receptor-T cell and T-cell receptor T-cell products, based on the droplet digital …
TCR sequencing paired with massively parallel 3′ RNA-seq …
2019年11月19日 · Here, we present a strategy that enables simultaneous analysis of TCR sequences and corresponding full transcriptomes from 3′-barcoded scRNA-seq samples. This …
Flow cytometry and IG/TCR quantitative PCR for minimal
A robust, reproducible, sensitivity of at least 0.01% has been achieved for IG/TCR clonal rearrangements using allele-specific quantitative PCR (IG/TCR-QPCR) within the EuroMRD …
TCR测序及分析方法简述 - 知乎 - 知乎专栏
t细胞介导的抗原识别取决于 t细胞受体 （tcr）与 抗原主要组织相容性复合体 （mhc）分子的相互作用。tcr是高度多样化的异源二聚体，由大多数t细胞表达的α和β链（αβ tcr）的组成，或由外 …
Quantification of circulating TCR-engineered T cells targeting a …
2024年9月12日 · The development of digital PCR has improved the accuracy in quantifying the in vivo status of adoptively infused T cells compared to qPCR or flow cytometry. Here, we …
精准前沿丨深度解读T细胞受体测序技术研究进展|TCR|细胞|方法|测 …
2022年5月16日 · 高通量测序技术的出现使研究人员第一次能够大批量对TCR序列进行平行分析，本文总结了将高通量测序技术和单细胞测序技术应用于TCR库的研究进展。 研究背景. T细 …