TCR测序及分析方法简述 - 知乎 - 知乎专栏
tcr是高度多样化的异源二聚体，由大多数t细胞表达的α和β链（αβ tcr）的组成，或由外周血中的t细胞（1-5%）和在粘膜部位发现的t细胞表达的γδ链（γδ tcr）组成。
单细胞免疫组库VDJ——基础知识（一） - 简书
2022年5月11日 · BCR和TCR有三个高变区CDR1、CDR2、CDR3，其中以CDR3变异最大，直定了抗原结合特异性，对于B细胞和T细胞多样性的研究也就集中在CDR3 区。 BCR的CDR3区 …
Entropic analysis of antigen-specific CDR3 domains ... - Cell Press
We present scanning parametrized by normalized TCR length (SPAN-TCR) to analyze antigen-specific TCR CDR3 sequences and identify patterns driving TCR-pMHC specificity. Using …
DeepAIR：有效整合序列和3D结构以实现TCR分析 - 健康界
2023年8月30日 · 结果表明，plddt总体上反映了tcr cdr3结构的预测精度。 使用具有不同pLDDT分数的预测CDR3结构的TCR来评估DeepAIR、DeepAIR-stru和DeepAIR-seq的AUC性能。 发 …
Overview of methodologies for T-cell receptor repertoire analysis
2017年7月10日 · Each TCR chain contains three hypervariable loops in its structure, termed complementarity determining regions (CDR1–3). CDR1 and 2 are encoded by V genes and …
The CDR3 regions of an immunodominant T cell receptor dictate …
2005年1月9日 · We quantified and evaluated the effect of natural and systematic substitutions in the complementarity-determining region (CDR) loops on ligand binding in the context of the …
CDR3 loop flexibility contributes to the degeneracy of TCR
2003年2月3日 · Comparison of these structures showed that the BM3.3 TCR complementarity-determining region (CDR) 3α could undergo rearrangements to adapt to structurally different …
A clonotype nomenclature for T cell receptors - PMC
To properly document and enumerate TCR CDR3, we have developed a working definition of a clonotype and a nomenclature that reflects the sequence information of the CDR3 of that …
Contribution of T Cell Receptor Alpha and Beta CDR3, MHC …
We improved it to create ERGO-II by adding the CDR3 alpha segment, the MHC typing, V and J genes, and T cell type (CD4+ or CD8+) as to the predictor. We then estimate the contribution …
Study of the T-cell receptor repertoire by CDR3 spectratyping
This technique consists in a CDR3 fragment analysis through capillary electrophoresis, performed after cell separation, RNA extraction and reverse transcriptase PCR. This review will run …