Engineering efficient termination of bacteriophage T7 RNA …
The termination hairpin found natively in the T7 genome is adapted to be inefficient, exhibiting 62% termination efficiency in vivo and even lower efficiency in vitro. In this study, we engineered a series of sequences that outperform the efficiency of the native terminator hairpin.
T7 promoter + terminators reporter plasmid - Addgene
A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
Plasmids 101: Terminators and PolyA signals - Addgene
2016年3月31日 · Nearly all common bacterial expression plasmids use Rho-independent terminators, which include naturally occurring terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators such as T0.
pQE-T7-1 Sequence and Map - SnapGene
Vector for expressing N-terminally 10xHis-tagged proteins in bacteria using the QIAgenes system. Sequence Author: Qiagen. Explore Over 2.7k Plasmids: Qiagen Vectors | More Plasmid Sets. Sticky ends from different DraIII sites may not be compatible. SmaI can be …
Sequence‐specific termination by T7 RNA polymerase requires …
2001年12月20日 · A series of transcription elongation complexes of T7 RNA polymerase were obtained and characterized at, and near, intrinsic, sequence-specific termination signals, via step-wise walking on DNA templates immobilized by biotin-streptavidin conjugation.
Characterization of two types of termination signal for bacteriophage ...
1994年4月29日 · The late bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3' G residue just downstream of the U run.
Sequence Analyzer: T7 promoter + terminators reporter plasmid …
To select a portion of sequence, click one location on the plasmid and then a second location to display the sequence between the two locations. Displays both strands of base paired nucleotide sequences with annotated enzymes, plasmid features, ORFs …
T7 promoter + terminators reporter plasmid Sequences (1)
Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).
Transcription termination in vitro by bacteriophage T7 RNA …
In this report, we demonstrate that the template deoxythymidine run upstream of the G+C-rich region, the loop-encoding segment, and the sequences upstream and downstream of the thr attenuator also affect termination.
Some DNA templates contain sequences that lead to the termination of transcription by T7 RNA polymerase. The include point substitutions in the G+C-rich region of dyad symmetry (12, 13, point substitutions in the A+T-rich.
T7 terminator (L1Y3QN) - Doulix
The T7 terminator is a sequence from bacteriophage T7 which allows efficient transcription termination.The bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3' G residue just downstream of the U run.
(PDF) Engineering efficient termination of bacteriophage T7 RNA ...
2021年12月9日 · The late bacteriophage T7 terminator (T7-T phi) encodes an RNA sequence that can form a stable stem-loop structure followed by a run of six uridylate residues; termination occurs at a 3' G...
Sequence-specific termination by T7 RNA polymerase requires …
Background: The sequence-specific, hairpin-independent termination signal for the bacteriophage RNA polymerases in Escherichia coli rrnB t1 terminator consists of two modules. The upstream module includes the conserved sequence and the downstream one is U-rich.
Unleashing The Power Of The T7 Terminator Sequence In …
2024年12月4日 · The t7 terminator sequence, a Rho-independent transcription termination signal, is crucial for controlling mRNA production in bacteria. This sequence influences mRNA stability, impact translation, and regulates gene expression. Its analysis has advanced DNA sequencing and genomic studies.
Engineering efficient termination of bacteriophage T7 RNA …
2022年5月30日 · The termination hairpin found natively in the T7 genome is adapted to be inefficient, exhibiting 62% termination efficiency in vivo and even lower efficiency in vitro. In this study, we engineered a series of sequences that outperform the …
Characterization of an Unusual, Sequence-specific Termination …
We have characterized an unusual type of termination signal for T7 RNA polymerase that requires a conserved 7-base pair sequence in the DNA (ATCTGTT in the non-template strand). Each of the nucleotides within this sequence is critical for function, as …
Transcription termination in vitro by bacteriophage T7 RNA …
1992年9月25日 · In this report, we demonstrate that the template deoxythymidine run upstream of the G+C-rich region, the loop-encoding segment, and the sequences upstream and downstream of the thr attenuator also affect termination.
(PDF) Engineering efficient termination of bacteriophage T7 RNA ...
2021年12月22日 · By embedding a previously discovered 8-nucleotide T7 polymerase pause sequence within a synthetic hairpin sequence, we observed in vivo termination efficiency of 91%; by joining two short...
T7 Promoter System - MilliporeSigma
What Is the T7 Promoter Sequence? The T7 promoter is a sequence of DNA 18 base pairs long up to transcription start site at +1 (5’ – TAATACGACTCACTATAG – 3’) that is recognized by T7 RNA polymerase 1 .
Is it necessary that there is a T7 terminator at the end of an insert ...
It is possible to do in vitro transcription directly using the plasmid if there is a terminator sequences. However, the transcription will bypass the T7 terminator in a percentage.
The Psu protein of phage satellite P4 inhibits transcription ...
2025年1月9日 · A DNA template for in vitro transcription 22, harboring a T7 RNAP promoter and the sequence encoding the λt R1 rut site, was produced from plasmid pUC18-λt R1-rut (Supplementary Table 4) by PCR ...
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