A User's Guide to the Arabidopsis T-DNA Insertional Mutant Collections 1. Q: Which Arabidopsis ecotype was used for the T-DNA collection? A: Columbia-0 (CS60000, the sequenced genome) 2. Q: Which T-DNA border flanking sequences were amplified and sequenced? What are the primers do you use for left border PCR?

T-DNA binary systems have greatly simplified the generation of transgenic plants. No longer are complex, sophisticated microbial genetic regimens required to integrate goi into T-DNA regions located on large, cumbersome Ti- or Ri-plasmids.

2004年8月1日 · Genetic transformation mediated by Agrobacterium involves the transfer of a DNA molecule (T-DNA) from the bacterium to the eukaryotic host cell, and its integration into the host genome.

In this chapter, we summarize our knowledge of the function of T-DNA bacterial and host protein chaperones, and draw a model for their action during the nuclear import and intranuclear transport of Agrobacterium T-DNA.

In this article we will discuss about the process of T-DNA transfer. Once plant exudates activating expression of vir genes and Agrobacterium attach to the plant cells, the T-DNA region in the Ti plasmid undergo excision process to transfer into the plant cells.

2017年11月27日 · Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir) effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir and plant proteins that traffic through the cytoplasm and enter the nucleus.

Agrobacterium has the ability to transfer T-DNA, a part of a tumor-inducing plasmid or Ti-plasmid, into the genome of many plants. A T-DNA binary system is a unique system, which was developed based on this mechanism. This system has been widely used in Agrobacterium -mediated plant transformation.

2016年10月31日 · Agrobacterium tumefaciens is a pathogenic bacterium, which transforms plants by transferring a discrete segment of its DNA, the T-DNA, to plant cells. The T-DNA then integrates into the plant...

2013年8月21日 · During plant genetic transformation, Agrobacterium transfers a single-stranded DNA (T-strand) into the host cell. Increasing evidence suggests that double-stranded (ds) T-DNA, converted from...

2016年10月31日 · Pol θ, an error-prone polymerase, can switch templates (usually to a nearby site), resulting in occasional filler DNA insertion (green double-stranded DNA) at the T-DNA integration site.