2013年8月25日 · Wapl depletion increased the mean Smc3-ChIP read density at the transcription start site (TSS) of many downregulated genes, including c-myc, and at non-TSS cohesin sites (Supplementary Fig. 13...

与此同时，SMC1-SMC3同时招募一个由Pds5和半翼蛋白(wings apart-like protein, Wapl)组成的异源二聚体（简称Pds5-Wapl），催化Rad21与SMC3之间的结合被拆开，这样一来粘合素环外门就打开，粘合素环因此也就被完全打开了(上图的“Rad21N-Psm3 open”步骤)。然后，粘合素环恢复 ...

2020年12月14日 · In this study, we used acute protein degradation to deplete WAPL in mouse embryonic stem cells (mESCs) to examine the immediate effects of changes in cohesin binding and 3D genome changes. We...

2014年7月17日 · Studies in fly and human have shown that this process involves the WAPL mediated opening of the cohesin ring at the junction between the SMC3 ATPase domain and the N-terminal domain of cohesin's α-kleisin subunit. We report here the isolation and detailed characterization of WAPL in Arabidopsis thaliana.

2020年3月23日 · WAPL promotes rapid RAD51-dependent repair and restart of broken replication forks. We propose that active removal of cohesin allows cancer cells to overcome DNA replication stress.

Following DNA replication in S-phase, Smc3 acetylation in the head domain promotes the recruitment of Sororin (11–14), which binds Pds5 and counteracts Wapl activity, thereby stabilizing a fraction of Cohesin on DNA to allow the establishment of sister chromatid cohesion .

2024年8月13日 · The ring-shaped Cohesin complex, consisting of core subunits Smc1, Smc3, Scc1, and SA2 (or its paralog SA1), topologically entraps two duplicated sister DNA molecules to establish sister chromatid cohesion in S-phase. It remains largely elusive how the Cohesin release factor Wapl binds the Cohesin c …

2023年9月22日 · We demonstrate that Smc3 acetylation blocks the association of Scc2 with pre-engaged cohesin by impairing the interaction of Scc2 with Smc3’s head. Lastly, we show that ATP binding induces the...

2009年3月27日 · Scc3, Pds5, and Rad61 (Wapl) are regulatory subunits that control this process. We describe here smc3, scc3, pds5, and rad61 mutations that permit yeast cell proliferation and entrapment of sister DNAs by cohesin rings in the absence of Eco1, an acetyl transferase normally essential for establishing sister chromatid cohesion.

2024年8月23日 · 本项研究利用一系列体内外蛋白互作实验，以及基于AlphaFold2的人工智能蛋白质结构分析，发现Wapl分别通过FGF基序和YNARHWN基序结合黏连蛋白核心亚基Scc1与SA2互作界面的不同区域（图3）。 破坏FGF基序或 YNARHWN基序与Scc1-SA2界面的结合，仅部分减弱Wapl与Scc1-SA2界面的结合，并部分削弱Wapl从染色体上移除黏连蛋白的能力。...