SirT1 antibody detects endogenous levels of total SirT1 protein. The antibody does not cross-react with other sirtuin proteins. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human SirT1. Antibodies are purified by protein A and peptide affinity chromatography.

60303-1-Ig targets SIRT1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

SIRT1通过催化关键蛋白的去乙酰化来调节细胞凋亡和肌肉分化等过程,在氧化应激和DNA损伤修复中发挥重要作用 (见图1)。 Sirt1的WB检测. 在 Western Blot 实验中，样本准备是实验成功的第一步。 1.裂解样本时加入足量的蛋白酶抑制剂防止蛋白降解。 2.该蛋白高度修饰，分子量达到130kd，可在转膜液中加入终浓度 0.1% 的 SDS 以提高转膜效率。 3.使用 Bradford，Lowry 或 BCA 法对总蛋白进行定量。 每个泳道加入 20-50μg 总蛋白，确保sirt1. 含量在 WB 检测限之上 …

SIRT1靶点介绍助您了解其蛋白功能，蛋白定位，蛋白特性及异构体和翻译后修饰。 同时列举了实用的WB实验小贴士，助您实验成功。

SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SIRT1.

Anti-SIRT1 is directed against human SIRT1. This product is an affinity purified antibody produced by immunoaffinity chromatography. Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.

SIRT1 Antibody. Applications: WB, IHC, ICC/IF, ELISA, IP. Reactivity: Human, Mouse, Rat, Non-human primate, Chicken. Publications: 367. Images: 189. Clonality: Polyclonal, Monoclonal, Recombinant Monoclonal. Conjugates: Unconjugated, Biotin, CoraLite Plus 670, FITC.

Browse primary antibodies for WB,... Antibodies that detect SIRT1 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA and Immunoprecipitation. These antibodies target SIRT1 in Human, Mouse, Rat, Non-human primate and Chicken samples.

SIRT1，也称为SIR2、SIR2L1和SIR2alpha，是sirtuin家族的重要成员，是一种NAD⁺依赖性脱乙酰酶，在调节各种细胞过程（包括转录调控、DNA损伤修复、细胞周期进展和衰老）中发挥着关键作用。 通过脱乙酰化关键底物（如肿瘤抑制蛋白p53），SIRT1调节了应激反应、代谢控制和长寿所涉及的重要途径，这使得SIRT1成为研究与年龄相关的疾病、癌症和代谢紊乱的重要靶标。 抗SIRT1抗体（B-7）具有广泛的物种反应性，可有效检测小鼠、大鼠和人类样本中的SIRT1， …