Romanowsky staining is a prototypical staining technique that was the forerunner of several distinct but similar stains widely used in hematology (the study of blood) and cytopathology (the study of diseased cells).

2022年6月23日 · Romanowsky Stains are the stains that are used in hematology and cytological studies, to differentiate cells in microscopic examinations of blood and bone marrow samples. These stains are also applied to detect the presence …

2023年11月21日 · Romanowsky stains are particularly effective at highlighting subtle differences in cellular morphology, aiding in identifying both normal and pathological conditions. The stains selectively color-specific cellular components, providing a dynamic and …

2024年5月19日 · Romanowsky staining, also known as Romanowsky–Giemsa staining, is a classic staining technique that paved the way for several different but similar stains widely used in haematology (the study of blood) and cytopathology (the study of …

Technical features of standardized and traditional Romanowsky stains are outlined, e.g., number and ratio of the acidic and basic dyes used, solvent effects, staining times, and fixation effects.

Romanowsky stains, such as Wright’s or Wright-Giemsa stains, are employed in diagnostic laboratories to stain blood smears. Rapid hematology staining kits that mimic the Wright stain are commonly used in clinical settings.

2024年12月18日 · These differences make Romanowsky stains versatile for general diagnostic purposes, while Wright stain excels in precision and detail, particularly in hematological contexts. Both stains are essential, each serving distinct yet complementary roles in laboratory medicine.

2024年7月10日 · In red blood cells, Romanowsky stains highlight morphological abnormalities that are essential for diagnosing hematological disorders. The stains bring out differences in cell size, shape, and color, which are indicative of conditions like anemia and spherocytosis.

We trace the history of cytopathological stains and discuss the advantages and limitations of Romanowsky-type stains for cytological evaluation. We also provide suggestions for the advantageous use of Romanowsky-type stains in cytopathology.

Romanowsky staining was an important methodological breakthrough in diagnostic hematology and cytopathology during the late 19 th and early 20 th centuries; it has facilitated for decades the work of biologists, hematologists and pathologists working with blood cells.