2024年2月19日 · Rho GTPases通过调节细胞骨架重塑、细胞迁移、细胞黏附、细胞极化和基因转录等过程参与细胞生物学的多个方面。在这篇综述中，作者提出并讨论了Rho GTPases的自组织能力，即直接从低阶状态过渡到高阶状态。也讨论Rho GTPase的自组织模式形成对细胞功能的优势。

2020年3月23日 · RhoGAPs are more promiscuous than RhoGEFs to confine Rho activity gradients. Our resource enabled us to uncover a multi-RhoGEF complex downstream of G-protein-coupled receptors controlling...

2016年6月15日 · Rho GTPases, which cycle between a GTP-bound active form and a GDP-bound inactive form, regulate cytoskeletal and cell adhesion dynamics and thus are crucial for the coordination of...

RHOGAP interacts with the switch I and II regions [3,133] and the P-loop of the RHO protein. The GAP domain accelerates the intrinsic GTP hydrolysis by RHO proteins in two ways. First, it directly contributes to catalysis by inserting catalytic arginine in the GAP domain into the active site of the RHO protein.

2024年1月3日 · Rho GTPases have what seems to be an excessively large repertoire of regulators: at least 80 different GEFs and 70 different GAPs 92 regulating the 3 most abundant GTPases — RHOA, RAC1 and...

In turn, Rho GTPases are regulated by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). To understand how endothelial cells initiate changes in the activity of Rho GTPases, and thereby regulate cell adhesion, we will discuss the role of Rho GAPs and GEFs in vascular biology.

Rho GTPase-activating proteins (RhoGAPs) can terminate Rho GTPases by stimulating their intrinsic rate of GTP hydrolysis. This rapidly converts Rhos to their GDP-bound state, and these membrane-bound proteins are then thought to be sequestered by guanine nucleotide dissociation inhibitors, allowing subsequent transport between membranes.

Three classes of regulatory proteins are involved in balancing Rho GTPases between the on and off states: the guanine nucleotide exchange factors (GEFs), which promote the release of bound GDP and facilitate GTP binding ; the GTPase-activating proteins (GAPs), which increase the intrinsic GTPase activity of Rho GTPases to accelerate the return ...

Rho GTP 酶是细胞骨架的中央调节因子，在人类中由 145 个多域鸟嘌呤核苷酸交换因子 (RhoGEF) 和 GTP 酶激活蛋白 (RhoGAP) 控制。 Rho 信号传导模式如何在动态细胞空间中建立以控制细胞形态发生尚不清楚。

2023年2月21日 · We show that a prometastatic RhoGAP, ARHGAP8/BPGAP1, binds to inactive Rac1 and localizes to lamellipodia. BPGAP1 recruits the RacGEF Vav1 under epidermal growth factor (EGF) stimulation and activates Rac1, leading to polarized cell motility, spreading, invadopodium formation, and cell extravasation and promotes cancer cell migration.