2022年4月13日 · Most RSV studies have involved either of two historic RSV/A strains infecting one of two cell lines, HEp-2 or A549 cells. However, RSV contains ample variation within two evolving subgroups (A and B), and HEp-2 and A549 cell lines are genetically distinct.

Most RSV studies have involved either of two historic RSV/A strains infecting one of two cell lines, HEp-2 or A549 cells. However, RSV contains ample variation within two evolving subgroups (A and B), and HEp-2 and A549 cell lines are genetically distinct.

2018年7月17日 · We show that A549 cells are permissive to RSV infection and express genes characteristic of a proinflammatory response. In contrast, BEAS-2B cells restrict infection and express genes characteristic of an antiviral response associated with expression of type I …

2022年3月14日 · The A549 cell line showed more instances of strain-dependence in its RSV response, suggesting heightened sensitivity to variation in infecting RSV strains. A549 cells also displayed an apoptotic response that rose monotonically to a peak at 96 hpi, while the HEp-2 apoptotic response was weaker and plateaued before dipping to a magnitude near ...

2021年6月16日 · Here we report a thorough molecular and cell biological characterization of HEp-2 and A549 cells infected with four strains of RSV representing both major subgroups as well as historic and more contemporaneous genotypes -- [RSV/A/Tracy (GA1), RSV/A/Ontario (ON), RSV/B/18537 (GB1), RSV/B/Buenos Aires (BA)] -- via measurements of viral ...

2020年8月1日 · Here, we established a simple and economic 3D spheroid culture of alveolar type II epithelial cells (A549) as a model to study RSV infection. This culture model can provide a tool for investigating the cellular and molecular mechanisms underlying RSV pathogenesis and to potentially test the effectiveness of antiviral molecules.

2018年1月10日 · In this study, we characterized exosomes released from RSV-infected airway-derived A549 cells and found that RSV induces significant changes in exosome RNA cargo composition, investigated...

2021年6月16日 · Here we measure viral action and host response in HEp-2 and A549 cells infected with four RSV strains from both subgroups and representing both historic and more contemporaneous strains. We discover a subgroup-dependent difference in viral gene expression and find A549 cells are more potently antiviral and more sensitive, albeit subtly, to ...

方法： 选用已经过全 基因组测序确定为RSV A亚型ON1基因型的地方株 (61397-ON1)感染A549细胞，提取总mRNA，通过转录组测序筛选出与未感染的A549细胞为对照的差异表达基因，对其进行GO分析、KEGG通路分析，同时随机选择6个差异表达倍数大于2倍的基因进行qRT-PCR验证。 结果： 以未感染的A549细胞为对照，筛选出1 632个差异表达基因，其中807个基因表达上调，825个基因表达下调。 结论： RSV A亚 型ON1基因型毒株感染A549细胞后的差异表达基因主要参 …

2022年11月23日 · We observed the interference between two prevalent respiratory viruses, respiratory syncytial virus (RSV) and influenza A virus (IAV) (H1N1), and characterized its molecular underpinnings in alveolar epithelial cells (A549). We found that RSV induces higher levels of interferon beta (IFN-β) production than IAV and that IFN-β priming confers ...