An optimized QF-binary expression system for use in zebrafish
2020年9月15日 · We optimized the Q binary expression system for zebrafish and other vertebrates. QFGal4 is a non-toxic transactivator that can be ubiquitously expressed in …
Formation of an RNA Quadruplex-Duplex Hybrid in Living Cells …
2020年10月29日 · Here, we present an effective novel strategy for inhibiting translation based on the antisense-controlled formation of an RNA quadruplex-duplex hybrid (QDH) between a G …
Separation of Plasmid DNA Topological Forms, Messenger RNA, …
2023年9月25日 · Modified mRNA (mRNA) represents a promising new class of therapeutic drug product. Development of robust stability indicating methods, for control of product quality, are …
Using Single Molecule mRNA Fluorescent - Nature
2018年5月21日 · We optimized a single molecule RNA fluorescence in situ hybridization (RNA-FISH) protocol, which amplifies fluorescence signal to detect candidate transcripts, for use …
IQ-Switch is a QF-based innocuous, silencing-free, and ... - Nature
2021年12月16日 · Here we demonstrate a novel QF-based binary transgene switch (IQ-Switch) that is relatively free of driver toxicity and transgene silencing, and exhibits potent and highly …
Adoption of the Q transcriptional regulatory system for zebrafish ...
QF is a transcriptional activator that binds to the QUAS upstream regulatory sequence to drive reporter gene expression. Unlike Gal4, the QF binding site does not contain essential CpG …
Schematic representation of Q-ASO and the EGFR mRNA
Recently, we have demonstrated the sequence-specific targeting of a 56 nt long EGFR mRNA fragment comprising two distant GGGG tracts by RNA oligonucleotides composed of a …
Qingfei xieding prescription ameliorates mitochondrial DNA …
2024年5月10日 · QF inhibited mtDNA-cGAS-cGAMP-STING pathway and subsequent inflammatory signaling via regulating the nuclear entry of P65, resulting in the alleviation in …
(PDF) Formation of an RNA Quadruplex-Duplex Hybrid in Living …
2020年10月29日 · Here, we present an effective novel strategy for inhibiting translation based on the antisense-controlled formation of an RNA quadruplex-duplex hybrid (QDH) between a G …
Models and melting curves of quadruplex-duplex hybrids (2ST …
Melting curves for QF-ASO/EGFR and QL-ASO/EGFR were monitored at 260 nm at 295 nm, in a buffer containing 50 mM KCl, 150 mM LiCl and 10 mM Tris-HCl at pH 6.8. from publication: …