pTT5-hSDC1 - Addgene
Plasmid pTT5-hSDC1 from Dr. Gordon Laurie's lab contains the insert SDC1 and is published in J Biol Chem. 2013 Apr 26;288 (17):12090-101. doi: 10.1074/jbc.M112.422717. Epub 2013 Mar 15. This plasmid is available through Addgene.
Protocol for high-throughput cloning, expression, purification, and ...
2022年6月17日 · The pTT5 vector is a suitable vector for both bacterial cloning as well as protein expression in mammalian hosts. It contains a CMV promoter to drive robust expression and an oriP cis -element to facilitate replication in human embryonic kidney (HEK) 293 …
Rapid generation of high-quality recombinant antibodies using an ...
2023年6月5日 · The pTT5 vector is often used to transiently express antibodies in mammalian cells, including the human cytomegalovirus CMV promoter. The presence of its upstream enhancer confers CMV the status of one of the most powerful known promoters among eukaryotic expression systems.
Part:BBa K3916000 - parts.igem.org
We aimed to construct the plasmid with the vector pTT5 to give the protein (S1&RBD) synthesized from B138 the ability to perform the protein purification and ELISA testing as well as to mimic the property held by the spike protein of the coronavirus.
Protein Expression and Purification Core Facility
PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. Overview of our mammalian expression vectors.
pTT5 (VET1182) - Creative Biogene
The pTT5 vector is a suitable vector for both bacterial cloning as well as protein expression in mammalian hosts. The prominent properties of this plasmid are the CMV promoter, the PMB1 ori replicon, and the beta globin poly (A) signal terminator, which enhance its ability to stimulate protein over-expression in the host system.
Protocol for high-throughput cloning, expression, purification, and ...
2022年6月1日 · The pTT5 vector is a suitable vector for both bacterial cloning as well as protein expres- sion in mammalian hosts. It contains a CMV promoter to drive robust expression and an oriP cis -
pTT5-hSDC1-2 - Addgene
Plasmid pTT5-hSDC1-2 from Dr. Gordon Laurie's lab contains the insert SDC1 and is published in J Biol Chem. 2013 Apr 26;288 (17):12090-101. doi: 10.1074/jbc.M112.422717. Epub 2013 Mar 15. This plasmid is available through Addgene.
To achieve high levels of gene expression, vectors such as pTT5 usually have strong promoters (e.g., the CMV promot-er) to drive expression of the antibody genes (Durocher et al. 2002).
A new method to customize protein expression vectors for fast ...
We modified expression vectors based on pET, pFastBac and pTT backbones for parallel PCR-based cloning and screening in E.coli, insect cells and HEK293E cells, respectively. We introduced the toxic ccdB gene under control of a strong constitutive promoter for counterselection of insert less vector.