Protofibrils of Amyloid-β are Important Targets of a Disease ... - PubMed
2020年1月31日 · We recently reported that protofibrils of Aβ 1-42 disturbed membrane integrity by inducing reactive oxygen species generation and lipid peroxidation, resulting in decreased membrane fluidity, intracellular calcium dysregulation, depolarization, and synaptic toxicity.
Protofibrils of Amyloid-β are Important Targets of a Disease …
We recently reported that protofibrils of Aβ 1-42 disturbed membrane integrity by inducing reactive oxygen species generation and lipid peroxidation, resulting in decreased membrane fluidity, intracellular calcium dysregulation, depolarization, and synaptic toxicity.
Protofibrils, the unifying toxic molecule of neurodegenerative ...
2001年9月1日 · A newly identified Alzheimer's mutation leads to the suggestion that protofibril intermediates in amyloid plaque formation may be a crucial factor in pathogenicity. Significant progress has...
Amyloid-β Protofibrils: Size, Morphology and Synaptotoxicity of
2013年7月2日 · Here we provide a detailed characterization of Aβ 42cc protofibrils. Like wild type Aβ they appear as smooth rod-like particles with a diameter of 3.1 (±0.2) nm and typical lengths in the range 60 to 220 nm when observed by atomic force microscopy.
Assembly of Aβ Amyloid Protofibrils: An in Vitro Model for a …
1999年6月18日 · Amyloid fibrils comprising primarily the peptides Aβ40 and Aβ42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway.
Protofibril–Fibril Interactions Inhibit Amyloid Fibril Assembly by ...
Metastable protofibrils, an alternative, particularly toxic, assembly type of amyloid proteins, bind amyloid fibril surfaces and obstruct secondary nucleation. Protofibrils thereby delay their own replacement by amyloid fibrils. Amyloid-β peptides (Aβ) assemble into both rigid amyloid fibrils and metastable oligomers termed AβO or protofibrils.
Protofibrils of Amyloid-β are Important Targets of a Disease
2019年12月31日 · We recently reported that protofibrils of Aβ 1-42 disturbed membrane integrity by inducing reactive oxygen species generation and lipid peroxidation, resulting in decreased membrane fluidity, intracellular calcium dysregulation, depolarization, and synaptic toxicity.
Structural Dynamics of Amyloid-β Protofibrils and Actions of Anti ...
2023年5月4日 · Amyloid-β (Aβ) aggregation intermediates, including oligomers and protofibrils (PFs), have attracted attention as neurotoxic aggregates in Alzheimer’s disease. However, due to the complexity of the aggregation pathway, the structural dynamics of aggregation intermediates and how drugs act on them have not been clarified.
Amyloid-β(1–42) Protofibrils Formed in Modified Artificial ...
2012年12月16日 · Here we describe the preparation and characterization of Aβ (1–42) protofibrils in modified artificial cerebrospinal fluid (aCSF) and demonstrate their prominent binding and activation of microglial cells.
Dynamics of protofibril elongation and association involved in …
2010年10月7日 · We discuss the challenges in estimating all the rate constants involved in the aggregation process towards fibril formation and propose a divide and conquer strategy by dissecting the pathway into three biophysically distinct stages: 1) pre-nucleation stage 2) post-nucleation stage and 3) protofibril elongation stage.