pLVX-Puro Sequence and Map - SnapGene
Lentiviral expression vector with the constitutive CMV promoter and a puromycin resistance marker. Sequence Author: Clontech (TaKaRa) Explore Over 2.7k Plasmids: Viral Expression & Packaging Vectors | More Plasmid Sets. The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
pLVX-M-puro - Addgene
Plasmid pLVX-M-puro from Dr. Boyi Gan's lab is published in Nat Cell Biol. 2018 Oct;20(10):1181-1192. doi: 10.1038/s41556-018-0178-0. Epub 2018 Sep 10. This plasmid is available through Addgene.
2010年7月1日 · pLVX-Puro Vector Map and Multiple Cloning Site (MCS). pLVX-Puro is an HIV-1-based, lentiviral expression vector. Lentiviral particles derived from the vector allow you to express your gene of interest in virtually any cell type, even primary cells.
pLVX-EGFP-IRES-puro - Addgene
Plasmid pLVX-EGFP-IRES-puro from Dr. Robert Sobol's lab contains the insert EGFP and is published in Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513. This plasmid is available through Addgene.
2nd and 3rd generation lentiviral packaging system and plasmids
2021年12月13日 · For most applications, you can produce viral particles by transient transfection of 293T cells with a 2nd generation packaging system (e.g.: packaging plasmid psPAX2 and envelop plasmid pMD2.G). Fig 1. Viral Production Schematic (from addgene.org)
pLVX-TetOne-Puro-GFP - Addgene
Plasmid pLVX-TetOne-Puro-GFP from Dr. Jason Sheltzer's lab contains the insert EGFP and is published in Dev Cell. 2021 Sep 13;56 (17):2427-2439.e4. doi: 10.1016/j.devcel.2021.07.009. Epub 2021 Aug 4. This plasmid is available through Addgene.
pLVX-Tight-Puro Sequence and Map - SnapGene
SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. The maps, notes, and annotations in the zip file on this page …
pLVX-TetOne-Puro Sequence and Map - SnapGene
pLVX-TetOne-Puro All-in-one lentiviral vector with a puromycin resistance marker, for strong inducible expression of genes using the Tet-On® system. Sequence Author : Clontech (TaKaRa)
pLVX-Puro vector map and sequence - novoprolabs.com
The pLVX-Puro is a lentiviral expression vector with the constitutive CMV promoter and a puromycin resistance marker. 1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4.
2008年9月26日 · pLVX-IRES-Puro is an HIV-1-based, lentiviral expression vector that allows the simultaneous expression of your protein of interest and puromycin resistance (Puror) in virtually any mammalian cell type, including primary cells.