pET-27b (+) pET27b质粒图谱、载体图谱、序列、价格、抗性、测 …
pET-27b载体带有N-pelB分泌信号肽标签，C-HSV标签和C-His标签。本载体与pet25b载体相比，只有抗性标记不同。独特的酶切位点在载体图谱中被标注出来。需要注意的是，这些载体序列是依据载体pBR322的方向来进行编号的，所以T7标签表达区域在载体图谱上是反向的。
pET-27b(+) Sequence and Map - SnapGene
Bacterial vector with a signal sequence and a kanamycin resistance marker for expression of C-terminally HSV-tagged proteins in the periplasm. Sequence Author : MilliporeSigma (Novagen) Open in SnapGene
pET-27b (+)_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研 …
The pET-27b (+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This vector differs from pET-25b (+) only by its selectable marker (kanamycin vs. ampicillin resistance).Unique sites are shown on the circle map.
Expression and Purification of Recombinant Proteins Using …
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in Escherichia coli Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals, expression is induced by providing a source of T7 RNA polymerase in the host cell ...
pET-27b(+) vector map and sequence - novoprolabs.com
The pET-27b(+) is a bacterial expression vector designed to produce C-terminally HSV-tagged proteins in the periplasm. It includes a signal sequence for proper protein targeting and a kanamycin resistance marker for selection.
pET vector. Plasmid for Expression by T7 RNA Polymerase. | PPT
2020年2月9日 · The pET vector system is a powerful tool for expressing cloned genes in E. coli. It utilizes the strong T7 promoter to drive high-level expression of the gene when induced. The T7 promoter is tightly regulated by the Lac repressor protein so expression is low without induction. This prevents toxicity from overexpression.
pET vector - | BDL・BioDynamics Laboratory Inc.
Vectors for protein expression in E. coli (T7 expression system). pET vector is an expression vector driven by T7 promoter. On the lineup of BDL, there are 3 types of vectors: high copy, medium copy, and vectors whose expression can be controlled by LacI. And each drug resistance marker can be selected from 2 types: ampicillin and kanamycin.
The pET Expression System - Davidson
To start the process, your favorite gene (YFG) is cloned into a pET plasmid at the polylinker site. Both the T7 promoter and the lac operator are located 5' to YFG. When the T7 RNA polymerase is present and the lac operator is not repressed, the transcription of YFG proceeds rapidly.
pET Bacterial Recombinant Protein Vector - VectorBuilder
2025年1月10日 · The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system. Activation of expression is achieved by providing T7 RNA polymerase within the cell.
pET vectors | PPT - SlideShare
2018年1月5日 · The gene for T7 RNA polymerase (gene 1) is inserted into the chromosome of E. coli and transcribed from the lac promoter; therefore, it will be expressed only if the inducer IPTG is added. The T7 RNA polymerase will then recognise the T7 promoter on the vector and transcribe the gene cloned into the pET vector. If the protein product of the ...