Our results suggest that the PERT assay is as sensitive as p24gag ELISA and gag PCR for detection of replication-competent HIV-1 in an RCL detection assay. Comparison of detection of replication-competent retroviruses, GALV and RD114, by extended S+/L- and PERT assays indicates that both assays can detect 1 IU of each virus.

但常规PERT 法是两步法RT-PCR加电泳检测,操作繁琐,误差大,不适合高通量. 操作。 为克服此缺点,本公司开发了一管式实时PERT试剂盒。 它具有下列特点: 即开即用,用户只需要提供待测样品。 操作简单快捷,一步加样操作,变异系数小,适合高通量操作。 实时荧光检测,一管式反应,避免样本交差污染。 灵敏性高,最低可以检测到几十个逆转录酶分子的活性。 周期中无DNA环节,不会自带污染源。 面所含有的逆转录酶活性,并由此判断是否有逆转录病毒的存在。 含逆转录酶阳性对照,便于 …

We present an evaluation of a modified SYBR Green I-based PERT assay (SG-PERT), using commercially available reagents such as MS2 RNA and ready-to-use qPCR mixes.

Here, we have assessed the performances of such a PCR-based RT assay--PERT assay--for the quantitative detection of RT activity in vaccines. Sensitivity, linearity and reproducibility of the method were studied on purified RT and viral vaccines treated to release RT from potentially contaminant retroviruses.

1999年10月1日 · PERT assays are RT-dependent PCR assays and therefore combine the broad specificity of conventional RT assays with the high sensitivity of PCR. As with conventional RT assays, they are used to detect the RT activity packaged into extracellular retrovirus particles.

未知逆转录病毒信息细胞系——对细胞进行电镜检测；上清进行逆转录酶活性测定（PERT）。 逆转录病毒阳性细胞——无需进行逆转录酶活性测定，但需要通过电镜检测确定逆转录病毒的类型（A型或C型），并选择合适的细胞进行感染性检测。

根据现有法规和指导原则可以发现，RCL的检测方法主要包含指示细胞培养法、ELISA法、PCR/q-PCR法和PERT法。 因为指示细胞培养法检测需28天，并且因为阳性对照病毒的性质，要求其需要在P3或者P2实验室环境中进行，致使该方法具有耗时长，环境要求高的特点。

Product Enhanced Reverse Transcriptase (PERT) assay has been used extensively to detect reverse transcriptase (RT) activity associated with retroviruses. We have assessed the usefulness of the PERT assay for RCL and RCR testing and compared it with existing assays for RCL (p24 gag ELISA/gag PCR) and RCR (S+/L-) detection.

Product safety testing for retroviruses can be achieved by a panel of screening assays, including electron microscopy, viral gene specific PCRs, virus propagation, and detection of reverse transciptase activity. The application of PCR-based reverse transcriptase assays (PERT) that are approximately …

Most qPCR based PERT assays use cDNA-specific fluorogenic labeled probes for signal generation (F-PERT). Recently, a one-step PERT assay using the more accessible and cost-efficient SYBR Green-I chemistry (SG-PERT) was also developed.