As markers for monocyte activation, differentiation, and induction of an innate immune response, expression of CD11b, TLR2, TLR4, and CD36 on THP1 cells was measured by flow (Fig. 1B), TLR2 (Fig....

2022年5月25日 · To determine whether THP-1 macrophages have larger amounts of LPS-related-receptors and intracellular proteins, CD14 and TLR4 were characterized by flow cytometry in THP-1 cells and PMA-differentiated THP-1 cells with or without LPS. Cell surface expression of CD14 and TLR4 was higher in PMA-differentiated THP-1 cells than in THP-1 cells.

In this study, we found that pro-inflammatory cytokines-IL-1β, IL-6 and TNFα showed greater induction in phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells than in THP-1 cells. To determine the difference in cytokine expression, the surface proteins such as TLR4-related proteins and intracellular adaptor proteins were more ...

2021年3月24日 · In vitro results show SYQP activated HEK-BLUE hTLR4 instead of HEK-BLUE Null cells. The secretion and the mRNA expression of cytokines related to TLR4 signaling significantly increased after SYQP treatment in both PMA-induced THP-1 and RAW264.7 macrophage cell lines. The TLR4 antagonist TAK-242 can …

2017年12月12日 · We found that THP1-derived macrophages or PMA primed THP-1 macrophages exhibit higher surface TLR4 levels and increased NOX2 expression levels following LPS treatment. M. tuberculosis infection reduced these levels, but LPS was able to limit the negative effects of M.tb .

(A) Expression of TLR2 and TLR4 in PMA-activated THP-1 cells was analyzed by RT-PCR. Total RNA was isolated from PMA-activated THP-1 cells cultured in medium with or without HK Y or H cells...

Neoseptins, chemically synthesized peptidomimetics that bind and activate the mouse TLR4 (mTLR4)/MD-2 complex independent of LPS, were discovered through unbiased screening and reverse genetic studies, and improved by chemical modification.

2016年10月10日 · Curcumin also markedly inhibited the upregulation of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), phosphorylation level of IκB-α, and activation of NF-κB in PMA-induced macrophages.

1.pma诱导hl-60细胞分化过程中tlr2和tlr4以及cd14的表达动力学研究;2.小鼠巨噬细胞热休克反应通过iκbα/nf-κb信号通路抑制lps诱导的白细胞介素12表达 来自 知网

2015年2月24日 · Our findings suggest that O. japonicus could elicit inflammatory reactions in PMA-activated THP-1 cells and contribute to inflammatory processes, a process mediated by TLR4 and, to a lesser extent, TLR2. The peptidoglycan subunits are recognized by the NOD family proteins, in particular by NOD2.