磷酸肌醇特异性磷脂酶 C (PLC) 在跨膜信号转导中扮演重要角色。 响应细胞外刺激，如激素、生长因子和神经递质刺激后，PLC 水解磷脂酰肌醇-4,5 二磷酸 (PIP2) 并产生第二信使肌糖 1,4,5 三磷酸 (IP3) 和甘油二酯 (DAG)。 至少已发现四种 PLCs 的家族成员：PLCβ、PLCγ、PLCδ 和 PLCε。 PLC 活动主要受到磷酸化调节。 例如，PLCβ3 在 Ser1105 位点通过 PKA 或 PKC 磷酸化后导致抑制性活动，而 PLCγ 在酪氨酸 771、 783 和 1245 位点通过受体 (EGFR) 和非受体的酪氨酸激 …

2000年6月2日 · We found that human PLD1 and PLD2 expressed in Sf9 cells can be activated by calcium-mobilizing agonists and by co-expression with PKCα. The calcium-mobilizing agonists A23187 and CryIC toxin triggered large increases in phosphatidylethanol (PtdEth) production in Sf9 cells over-expressing PLD1 and PLD2, but not in vector controls.

2020年4月7日 · Chemotaxis of fibroblasts and other mesenchymal cells is critical for embryonic development and wound healing. Fibroblast chemotaxis directed by a gradient of platelet-derived growth factor (PDGF) requires signaling through the …

Delayed Phosphorylation of Classical Protein Kinase C (PKC) Substrates Requires PKC Internalization and Formation of the Pericentrion in a Phospholipase D (PLD)-dependent Manner *

1996年5月24日 · In nearly all mammalian cells and tissues examined, protein kinase C (PKC) has been shown to serve as a major regulator of a phosphatidylcholine-specific phospholipase D (PLD) activity. At least 12 distinct isoforms of PKC have been described so far; of these enzymes only the alpha- and beta-isoform …

1999年7月30日 · First evidence that PKC was involved in PLD activation derived from the observation that phorbol esters increased PLD activity. In cell-free systems, using recombinant PKC isoforms and membranes derived from human neutrophils, the conventional PKCs significantly upregulated PLD activity [64].

2004年5月21日 · In the present study it was demonstrated that phorbol 12-myristate 13-acetate (PMA) induced PLD2 activation in COS-7 cells. PLD2 was also phosphorylated on both serine and threonine residues after PMA treatment. PKC inhibitors Ro-31-8220 and bisindolylmaleimide I inhibited both PMA-induced PLD2 phosphorylation and activation.

2021年1月1日 · Mammalian phospholipase D (PLD) generates phosphatidic acid, a dynamic lipid secondary messenger involved with a broad spectrum of cellular functions including but not limited to metabolism, migration, and exocytosis. As a promising pharmaceutical target, the biochemical properties of PLD have been well characterized.

2020年3月16日 · Phospholipase D (PLD) regulates various cellular functions through the signaling lipid phosphatidic acid (PA). New crystal structures of human PLD1 and PLD2 reveal a...

PLD信号通路是细胞内的一种次级信号传递系统，通过PLD代谢产物——PA的作用，参与调控多种生物学过程，包括细胞分化、增殖、运动、内质网应激反应以及膜结构、细胞架构的改变等。