Here we investigated whether the Janus kinase-signal transducer and activator of transcription (JAK-STAT) pathway contributes to platelet-derived growth factor (PDGF)-stimulated mitogenesis of human airway smooth muscle cells (HASMC). PDGF treatment of quiescent HASMC resulted in the rapid tyrosine phosphorylation and DNA binding of STAT1 and ...

2023年5月1日 · PDGF-BB and PDGF-DD can trigger JAK/STAT1/3 cell signaling pathway via PDGFRβ. In 293 T and COS-7 renal cells, binding of Tyr581 and Tyr579 residues (of activated PDGFRβ) to SH2 domain (of STAT5) does not disturb …

In this study, we examined the role of JAK-STAT pathway in PDGF-induced proliferation of PSCs. We showed here that PDGF induced the activation of Src, JAK2, STAT1, and STAT3 in PSCs. JAK proteins phosphorylate STAT proteins at tyrosine residues and activate them, although other mechanisms might be involved[ 28 , 29 ].

2022年3月31日 · PDGF-DD has been demonstrated to activate the PI3K/AKT signaling in bladder cancer cells through PDGFRβ. PDGF-DD is also found to promote proliferation, survival, and migration of vascular cells and fibroblasts by activating PDGFRβ and the downstream AKT phosphorylation [27].

The PDGF-induced phosphorylation of both JAK and STAT requires the intrinsic kinase activity of PDGFR, suggesting that both the JAK activation mechanism and its function, if any, differ for cytokine receptors (Figure 2C) [12,38,60].

2024年11月14日 · PDGF-AB can combine with PDGFRα to activate the signal transducer and activator of transcription (STAT) signaling pathway through the action of the Janus kinase (JAK) and sarcoma enzyme, and upregulate the expression of cyclin D1 and Myc to promote cell proliferation in human airway VSMCs (Figure 1a) .

Since certain cytokines activate STATs via tyrosine phosphorylation mediated by JAK family of tyrosine kinases, we studied the effect of PDGF on JAK kinases. Antiphosphotyrosine immunoblotting of JAK 1 immunoprecipitates from PDGF-stimulated mesangial cell lysate showed increased tyrosine phosphorylation of this tyrosine kinase.

1999年7月14日 · Activation of the platelet-derived growth factor (PDGF) receptor tyrosine kinase induces tyrosine phosphorylation of Signal Transducer and Activator of Transcription (STAT) proteins. Since the...

2020年7月9日 · Bioinformatics analysis identified that PDGF-BB/PDGFRβ signals were relevant to the activation of JAK/STAT3 signals. The protein expression of JAK2 and STAT3 was elevated in PDL of tension side. Importantly, in vivo, the treatment of the inhibitors (imatinib and AG490) for PDGFRβ and JAK-STAT signals were capable of attenuating the tooth ...

2024年7月1日 · After dimerization and autophosphorylation, PDGFR can activate downstream molecules containing a SH2 domain, including PI3K, MAPK, PLCγ and JAK, which finally cause cell proliferation, survival and migration. The PI3K/AKT pathway is one of the key downstream pathways of BCR-ABL.