Multiplex PCR-restriction fragment length polymorphism appears to be a useful and convenient method for rapidly detecting and discriminating genotypes for vancomycin-resistant Enterococcus spp. in the clinical laboratory.

In terms of detecting VRE at the hospital, VRE can be diagnosed by a few standard methods such as antibiotic susceptibility, molecular diagnosis assays, and polymer chain reaction (PCR) using human samples of blood, urine, pus, or other fluid from the infected area . Detection of VRE

2020年10月1日 · In this study, we evaluated the performance of quadruple real-time PCR to detect vanA, vanB and vanM gene. A total of 343 strains, including 253 vancomycin-resistant Enterococcus faecium strains and 90 non-VRE strains, were collected by Institute of clinical pharmacology, Peking University First Hospital.

2021年5月1日 · Overall, 246 isolates of enterococci were collected from various clinical specimens. The isolates were identified, and antimicrobial susceptibility testing was done using the Vitek 2 system. Multiplex PCR was performed on the VRE isolates, thus identified to determine the van genes harbored.

Conclusion: PCR is a more rapid and sensitive assay for simultaneous detection and characterization for Enterococcus faecalis, and determination of its sensitivity pattern to vancomycin. Key Words: Enterococcus faecalis, multiplex-PCR, Van A, Van B.

Multiplex polymerase chain reaction (PCR) was used to detect the presence of van genes. Results: Out of all the clinical and fecal samples processed, 12.0% isolates were either vancomycin resistant or vancomycin intermediate. Further, these isolates carried van A or van B genes as confirmed by PCR methods.

2022年12月2日 · Genes encoding vancomycin resistance were studied by multiplex-PCR, and susceptibility to six antibiotics was evaluated by disk-diffusion method. In total, 107 Enterococcus spp. isolates were obtained and confirmed by biochemical and molecular tests.

Considering the excellent PPV and low NPV, real-time PCR would be useful to monitor VRE-colonized or infected patients. However, further evaluation of the assay's performance in the clinical specimens is required, especially when considering that high level of PCR inhibitors present in these samples …

2018年2月1日 · In this report, we have developed new colony multiplex PCR assays for detection and discrimination of seven types of vancomycin determinants (vanA, vanB, vanC1, vanC2 / 3, vanD / D4, vanM and vanN) and eight Enterococcus spp. (E. faecalis, E. faecium, E. gallinarum, E. casseliflavus / E. flavescens, E. raffinosus, E. avium, E. hirae and E. duran...

2025年3月24日 · Two PCR assays were developed for rapid outbreak detection: a traditional PCR assay distinguishing outbreak from non-outbreak strains based on amplicon size and a TaqMan real-time PCR assay. Both assays demonstrated 100% reproducibility and specificity. ... The activated VanR binds to the promoter regions of the van operons (vanA or vanB ...