2013年8月1日 · The LIR motif of p62 presents as an extended β-strand that forms an intermolecular parallel β-sheet with the β2 strand of LC3B. The ATG8 family proteins have a C-terminal, ‘core’ Ubl domain that contains the conserved ‘ubiquitin fold’ and an additional N-terminal arm with two α-helices that are closed onto the core Ubl domain ( Fig ...

2019年5月3日 · Modulating LIR binding specificity of the centriolar satellite protein PCM1, implicated in autophagy and centrosomal function, alters its dynamics in cells. Our data provides new mechanistic...

研究发现，p62主要通过其UBA（Ser403位点）结构域、LIR（Ser351位点）结构域的磷酸化，分别与泛素化蛋白和 LC3/Atg8 连接，同时通过 PB1 发生自身寡聚化，最终进入自噬溶酶体完成泛素化底物降解。

2020年1月3日 · A region in p62 encompassing amino acids 326–380 is sufficient for this interaction and defines the FIP200-interacting region (FIR). This region also contains the LIR and KIR motifs. The binding of p62 to LC3B or FIP200 is mutually exclusive [167].

2021年1月4日 · In this report, we describe the properties of endogenous p62-bodies, the effect of autophagosome biogenesis on these bodies, and the in vivo significance of their turnover. p62-bodies are...

摘要：螯合体1 (SQSTM1/p62)是一种选择性自噬接头蛋白，在清除待降解蛋白、维持细胞内蛋白质稳态中发挥重要的调控作用。 p62蛋白具有多个功能结构域，介导与多种蛋白质发生相互作用进而精确调节特定的信号通路，从而将p62蛋白与氧化防御系统、炎症反应和营养感知等重要生命过程联系起来。 研究表明p62的突变或者表达异常与多种疾病的发生发展过程密切相关，包括神经退行性疾病、肿瘤、感染性疾病、遗传性疾病以及慢性疾病等。 本文综述了p62蛋白的结构特征 …

2025年2月25日 · The phase separation of the cargo receptor sequestome-1/p62 (SQSTM1/p62) is a critical mechanism for assembling signaling complexes in autophagy. During this process, p62 undergoes phase separation upon binding to polyubiquitin chains, concentrating ubiquitinated substrates within p62 droplets.

The role of p62 as a cargo receptor in autophagy is mediated by the LIR motif, linking it to LC3 on autophagosomes, and the UBA domain, which binds to ubiquitinated cargos. Upon phosphorylation at Ser349, the KIR motif interacts with Keap1, thereby liberating Nrf2 and inducing Nrf2 target gene expression.

2007年8月17日 · Mapping of the LC3 Interacting Region (LIR) of p62—To further map the LC3 interaction region, we made a series of deletion constructs of GFP-tagged p62 that were translated in vitro in the presence of [35 S]methionine and subjected to GST pulldown experiments with GST-LC3B bound to glutathione-Sepharose …

p62 is a multidomain protein that contains different kinds of protein-protein interaction domains, including an N-terminal PB1 domain, a ZZ-type zinc finger domain, a nuclear localization signal (NLS), an export motif (NES), the LC3-interacting region (LIR), the KEAP1-interacting region (KIR), and a C-terminal Ub-associated domain (UBA). p62 is ...