Recombinant p62 does not undergo phase separation in vitro; however, adding a K63 polyubiquitin chain to p62 induces p62 phase separation, which results in enrichment of high-molecular weight ubiquitin signals in p62 droplets.

2018年3月5日 · Recombinant p62 does not undergo phase separation in vitro; however, adding a K63 polyubiquitin chain to p62 induces p62 phase separation, which results in enrichment of high-molecular...

2020年12月18日 · The scaffold protein p62 binds K63-linked ubiquitin chains and has been shown to play a crucial role in Parkin-facilitated mitochondrial clustering in the perinuclear region 15,16.

由于 p62 可以通过其 UBA 结构域与 多聚泛素化链 结合（ p62 小体包含 K63 多聚泛素化链），并且多价的相互作用能够促进相分离的发生，所以 作者推测多聚泛素化链与 p62 之间的多价相互作用可能促进 p62 的相分离。

Here we present compelling evidence demonstrating a role for the p62/TRAF6 complex in the amino acid-mediated activation of mTORC1 by K63-type polyubiquitination of mTOR. We have demonstrated that interfering with mTOR’s ubiquitination or with the p62-TRAF6 interaction impairs mTORC1 activation in response to nutrients and has an important ...

2022年5月2日 · Parasitophoric vacuoles containing type II Toxoplasma gondii are ubiquitinated by increased Interferon γ levels, mostly by K63-linked chains, which is also recognised by p62 and NDP52. In this process, the vacuoles containing pathogens are directly fusing with lysosomes, without additional autophagosome formation (Clough et al. 2016 ).

2016年3月3日 · Here we show that TRIM21 plays an essential role in redox regulation by directly interacting with SQSTM1/p62 and ubiquitylating p62 at lysine 7 (K7) via K63-linkage. As p62 oligomerizes and sequesters client proteins in inclusions, the TRIM21-mediated p62 ubiquitylation abrogates p62 oligomerization and sequestration of proteins including Keap1 ...

2021年7月13日 · We found that the ISGylation of TRIM21 induced by IFN- β elevates the levels of K63-linked ubiquitination of TRIM21 as well as p62 by enhancing its E3 ubiquitin ligase enzymatic activity...

HDAC6 can be activated by the products in UPS (such as K63), but inhibited directly by p62. HDAC6 plays a critical role in ubiquitinated aggregate formation and autophagosome–lysosome fusion, while a ratio of p62 to HDAC6 maintains the homeostasis of autophagic process.

Substrate‐attached linear, K48‐, and K63‐linked ubiquitin chains were all able to trigger cluster formation by p62. K63‐linked chains were more potent than K48‐linked chains under the conditions tested, implying that clustering in the absence of proteasomal inhibition could be specifically triggered by K63‐linked chains, while ...