2016年6月27日 · Phosphorylated p62/Sqstm1 accumulates in tumour regions positive for hepatitis C virus (HCV). An inhibitor of phosphorylated p62-dependent Nrf2 activation suppresses the proliferation and...

2017年6月15日 · Our findings show how p62 helps maintain intracellular pools of GSH needed to limit mitochondrial dysfunction in tumor cells with elevated mTORC1, highlighting p62 and redox homeostasis as nodal vulnerabilities for therapeutic targeting in these tumors.

2012年8月1日 · In our study, we found that HT increases p62 mRNA and protein levels dose dependently; we also found that regulation of p62 accumulation is not involved in autophagy. p62 expression and GSH content were decreased by JNK inhibitor SP600125 treatment.

2025年2月1日 · Mechanically, SFN directly targeted p62 protein and enhanced p62/SLC7A11 protein-protein interaction, thereby promoting the lysosomal degradation of SLC7A11 and triggering ferroptosis. Notably, both subcutaneous and intratibial OS models in nude mice confirmed the ferroptosis associated anti-cancer efficacy of SFN in vivo .

SQSTM1/p62 (sequestosome 1) is a multifunctional signaling molecule, involved in a variety of cellular pathways. SQSTM1 is one of the best-known autophagic substrates, and is therefore widely used as an indicator of autophagic degradation. Here we report that the expression level of SQSTM1 can be restored during prolonged starvation.

2017年6月6日 · Metabolic profiling revealed that depletion of p62 in Tsc2-null cells decreased intracellular glutamine, glutamate, and glutathione (GSH). p62 positively regulated the glutamine transporter Slc1a5 and increased glutamine uptake in Tsc2-null cells.

SQSTM1/p62 是由SQSTM1基因编码的一种多功能泛素化结合的接头蛋白,参与泛素蛋白酶体系统和自噬-溶酶体系统两种蛋白质降解过程,同时具有质核穿梭功能,在DNA损伤修复和氧化应激反应中发挥重要作用。 p62作为细胞接头蛋白,是细胞内多种蛋白复合物的主要成分[1],其分子结构. 细胞的生长、自噬、癌变等生理病理过程。 p62不仅是自噬体与底物之间的适配蛋白,反过来也调节着自噬的过程。 当自噬活性减弱时,p62蛋白会在细胞质中累积,是反映自噬活性的标记蛋白之一[5]。 …

2021年11月16日 · We found markedly increased hepatic levels of p62 at 24 h after APAP treatment, which is correlated with increased NRF2 target gene expression and hepatic GSH recovery. In contrast, p62 KO mice had decreased NRF2 activation and delayed hepatic GSH recovery after APAP exposure.

5 天之前 · By delineating how ferroptosis markers—Fe 2*, MDA, GSH, and GPX4—are regulated in tandem with p62/Nrf2 activation, we demonstrate a previously unrecognized convergence between microglial inflammatory responses and ferroptotic vulnerabilities in the context of PD. Furthermore, through molecular docking and mouse model studies, we have ...

CDGSH 铁硫结构域 2 (CISD2) 是一种具有 [2Fe-2S] 簇的铁硫蛋白，对于细胞增殖和铁稳态至关重要。 已证明 CISD2 的异常表达与多种癌症的进展相关。 然而，CISD2 调节肿瘤发生的潜在机制仍不清楚。 采用生物信息学策略研究 CISD2 的蛋白质相互作用网络和功能注释。 功能实验中，采用CCK-8试剂盒测定细胞活力。 分别通过 DCF-DA、RPA、C11-BODIPY 和组织蛋白酶 B 染色测定细胞活性氧 (ROS)、细胞内游离铁、脂质过氧化物和溶酶体活性的水平。 使用 GSH 测定试剂 …