2019年12月19日 · Here we show that under nutrient-deficient conditions, cellular p62 specifically undergoes acetylation, which is required for the formation and subsequent autophagic clearance of p62 bodies. We...

2023年10月5日 · In cells, p62 promotes the assembly and removal of ubiquitinated proteins by forming insoluble cytoplasmic inclusions known as p62 bodies. 21 p62 bodies have liquid-like properties formed by polyubiquitin chain-triggered liquid-liquid phase separation, 24, 25 which is required for p62 body formation and p62-mediated selective autophagy. 26, 27 ...

2020年12月14日 · Data from SCC-25 cells demonstrated that rapamycin and Earle’s balanced salt solution (EBSS) promoted autophagy, leading to increased FN1 degradation, while 3-methyladenine (3-MA), bafilomycin ...

2024年7月29日 · Regulation of autophagy through the 62 kDa ubiquitin-binding protein/autophagosome cargo protein sequestosome 1 (p62/SQSTM1), whose level is generally inversely proportional to autophagy, is crucial in microglial functions. Since autophagy is involved in inflammatory mechanisms, we investigated the …

The autophagosome merges with the lysosome where sequestosome-1 (commonly known as p62) incorporates into autophagosomes and degrades LC3II, along with additional cargo proteins (51).

2017年3月21日 · Ubiquitylation of p62 disrupts dimerization of the UBA domain of p62, liberating its ability to recognize polyubiquitylated cargoes for selective autophagy. We further demonstrate that this...

In this study, we set out to localize and quantitatively analyze cytoplasmic p62/SQSTM1 levels together with p62/SQSTM1 immunoreactive puncta of p62/SQSTM1 in various pro- and anti-inflammatory settings using bacterial lipopolysaccharide (LPS) and RST, respectively, in the presence or absence of BAF in secondary microglial cultures.

2016年6月1日 · Data provided include analyses of protein levels of LC3 and p62 by Western-blotting and endogenous immunofluorescence experiments, but also p62 mRNA levels obtained by quantitative PCR (qPCR). To monitor the turnover of these autophagy markers and, thus, measure the flux of this pathway, cells were under starvation conditions and/or treated ...

In this study, we report that host cargo receptor protein p62 selectively targets DENV-2 C protein for autophagic degradation, and this process was enhanced by autophagy activation, such as mTOR inhibitor treatments.

在自噬体形成过程中，p62作为链接LC3和聚泛素化蛋白之间的桥梁，被选择性地包裹进自噬体，之后被自噬溶酶体中的蛋白水解酶将其降解（图9），所以p62蛋白的表达量与自噬活性呈现负相关。