Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb detects endogenous levels of IRF-3 when phosphorylated at Ser396. The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has …

在STING发现以前，大量的研究已经表明两类重要的转录因子（transcription factors），一个是NF-kB，另一个是IRF3，这两个转录因子都能在病毒入侵时诱导一系列基因表达谱发生改变以产生病毒抵抗。

IRF3 (Interferon regulatory factor 3) 作为干扰素调节因子的一员，在抗病毒天然免疫防御方面发挥重要作用。IRF3 可以调节IFN和IFN诱导基因（ISG）的表达。 干扰素调节因子家族包括 9 个成员。IRF3 作为细胞抗病毒反应的核心转录因子，在各组织细胞中组成型表达。

3 天之前 · Phospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members at physiological conditions.

IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation.

irf3 在先天免疫系统对病毒感染的反应中起着重要作用。 聚集的MAVS 可以激活 IRF3 的二聚体。 2015 年的一项研究显示，先天免疫适应蛋白 MAVS、STING 和 TRIF 在一个保守的pLxIS 图案上的磷酸化可以招募并指定 IRF3 的磷酸化和被丝氨酸/苏氨酸-蛋白激酶TBK1 激活，从而 ...

Phosphorylated (P-) IRF3 subsequently translocated into nucleus and increased the expression of NOD-like receptor protein 3 (NLRP3). Knockout of STING in mice significantly improved survival rate and cardiac function, apart from suppressing myocardial and serum inflammatory cytokines, apoptosis, as well as cardiomyocyte pyroptosis.

IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites.

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster.

构建野生型irf3（irf3-wt）和irf3-5d过表达的结直肠癌细胞ct26、colon26，采用细胞计数法、细胞划痕试验和克隆形成试验检测细胞增殖及迁移能力。 结果·TCGA数据分析提示癌组织中IRF3蛋白表达水平与患者的不良预后呈正相关。