2022年11月14日 · Oxygen transport is fundamental to aerobic respiration and the survival of complex organisms. The lungs, heart, vasculature, and red blood cells play essential roles in oxygen transport.

Cells have a wide range of oxygen utilization, depending on cell type, function, and biological status. Measured rates of oxygen utilization by mammalian cells in culture range from <1 to >350 amol cell -1 s -1.

Here, we describe a novel in vitro system that utilizes hypoxic chambers and oxygen-permeable culture dishes to control pericellular oxygen levels and provide rapid oxygen delivery to adherent cells. This procedure is particularly relevant for protocols studying effects of rapid oxygen changes or intermittent hypoxia on cellular behavior.

Oxygen availability is often a limiting factor for cell survival, and it is generally supplied to a cell by passive diffusion. As oxygen molecules diffuse into the cell, they are consumed, so that there is a progressive fall in oxygen concentration from the surface of the cell to the lowest concentration which occurs at the center of the cell.

For purposes of discussing oxygen transport by the blood, we will consider blood to be composed of two phases: plasma and red blood cells (RBCs). The fractional volume of blood occupied by RBCs is called the hematocrit, and its value is a little less than 50% in human adults (∼40% for females and ∼45% for males).

2025年1月1日 · Discuss the implications of poor oxygen control on cellular O2 regulation mechanisms, bioenergetics, and redox signaling. Proposes and updated methodology on best practices for oxygen-controlled cell culture.

Oxygen is the second most abundant element in Earth's atmosphere. Although critical for aerobic respiration, it also poses significant dangers to life. The reduction of molecular oxygen to...

O2 sensing is a fundamental biological process necessary for adaptation of living organisms to variable habitats and physiological situations. Cellular responses to hypoxia can be acute or chronic. Acute responses rely mainly on O2-regulated ion channels, which mediate adaptive changes in cell excit …

2020年5月23日 · Molecular oxygen (O 2) is fundamental for cellular bioenergetics and metabolism and thus a critical factor in the normal function and survival of most multicellular organisms. As such, essential mechanisms have been developed in all aerobic multicellular species to sense and respond to changes in O 2 levels.

Oxygen transport within the human body occurs through both convection and diffusion. Within the pulmonary capillaries, one haemoglobin molecule binds up to four oxygen molecules in a cooperative manner.