2006年12月29日 · The results show that p53 counteracts the Nrf2-induced transcription of three ARE-containing promoters of the x-CT, NQO1, and GST-alpha1 genes. Endogenous transcripts of these antioxidant genes accumulate as a consequence of Nrf2 overexpression or exposure to electrophile diethylmaleate, but these effects are again blocked by p53 overexpression ...

2017年10月5日 · Here, we identified ARF as a key regulator of nuclear factor E2-related factor 2 (NRF2) through complex purification. ARF inhibits the ability of NRF2 to transcriptionally activate its target genes, including SLC7A11, a component of the cystine/glutamate antiporter that regulates reactive oxygen species (ROS)-induced ferroptosis.

Silencing of NRF2 abolished mutant p53 ability to sustain TXN and TXNRD1 mRNA levels indicating that mutant p53 requires NRF2 to promote the transcription of these genes. Since NRF2 plays a major role as an antioxidant response regulator in cancer and in normal cells we sought to test if the regulation of the expression of NRF2 targets by ...

2012年12月15日 · We observed a two-phase Nrf2 expression controlled by p53. (i) The induction phase: when p53 expression is relatively low, p53 enhances the protein level of Nrf2 and its target genes to promote cell survival in a p21-dependent manner. (ii) The repression phase: when p53 expression is high, the Nrf2-mediated survival response is inhibited by p53.

We observed a two-phase Nrf2 expression controlled by p53. (i) The induction phase: when p53 expression is relatively low, p53 enhances the protein level of Nrf2 and its target genes to promote cell survival in a p21-dependent manner. (ii) The repression phase: when p53 expression is high, the Nrf2-mediated survival response is inhibited by p53.

p53 Overexpression Interferes with the Nrf2-mediated Induction of ARE-containing Gene Promoters—The x-CT gene transcription is activated by the electrophilic agent DEM, through a mechanism based on the recruitment of Nrf2 transcription factor on the four AREs present in the 235 bp upstream of the transcription start site (Fig. 1A).

2016年12月16日 · A quantitative dynamics pathway map of the Nrf2-mediated oxidative stress response and p53-related DNA damage response pathways as well as the cross-talk between these pathways has not systematical...

2017年10月5日 · Our data demonstrate that NRF2 acts as an important target of ARF and that ARF-mediated repression of NRF2 is critical for both the apoptotic and ferroptotic responses under oxidative stress in p53 null cells.

2024年2月1日 · During cell cycle arrest at G 2 phase, p53 expression in IDH-wildtype glioblastoma in elderly individuals, coupled with the downregulation of NDRG2 gene activity, led to an aberrant increase in tumor cell proliferation and accelerated tumor recurrence. However, the influence of p53 on NRF2 gene activity was found to be insignificant. 1.

2023年10月10日 · In phase one, when the level of OS in the cell is low, p53 increases cell survival through the p21 pathway by increasing the expression of Nrf2, but phase two is when the level of OS in the cell is high, and p53 increases apoptosis by decreasing the expression of Nrf2.