Isolated colonies of L. monocytogenes on BHI agar are small (~1 mm), creamy white in color, and dome-shaped. Introduce the inoculum into sterile BHI broth in a culture flask or tube by gently swirling the loop within the broth.

琼脂糖是一种热可逆的离子依赖性胶凝剂，在水中通常加热至90℃以上融解，温度下降到35-40℃时形成良好的半固体状的凝胶，因此在分子生物学中常用于DNA或RNA的凝胶电泳分析和切胶回收 [1-2]。 低熔点琼脂糖是一种改性后具有独特胶凝性能的琼脂糖，相比于常规琼脂糖，其凝胶温度和熔点更低，凝胶的透明度更优，分子筛特性更好，电泳条带清晰度更高，分辨率极高，特别适用于大于1000bp核酸的凝胶电泳分离及回收，以及制备含有热不稳定材料的培养基等 [3]。 …

For solid NGM, mix 3 g of NaCl, 2.5 g of peptone, and 20 g of agar and bring to 1 L with H 2 O. (For liquid NGM, prepare without agar.) 2. Autoclave for 1 h. 3. Let cool for 1 h in a 55°C water bath. 4. Add 1 mL of cholesterol (5 mg/mL in ethanol), 1 mL of 1 m CaCl 2, 1 mL of 1 m MgSO 4, and 25 mL of 1 m (pH 6.0) KPO 4, mixing after each addition

2024年10月16日 · In this study, 2-(4-bromo-2-chlorophenoxy)-N-(2,4-dimethoxyphenyl) acetamide (NMA) was identified by screening a small-molecule chemical library that induced abnormal leaf vein patterns in the cotyledons of Arabidopsis thaliana. NMA promoted hypocotyl elongation, inhibited primary and lateral root growth under light condition.

The slides are covered with 110 microliters at 1% normal melting point agarose (NMA) dissolved in buffer (1 x PBS) at 45 ° C. It is then covered with a coverslip and held at 4 ° C for 10 min to...

2019年2月6日 · Here we show that SznF, a metalloenzyme from the biosynthesis of streptozotocin, catalyses an oxidative rearrangement of the guanidine group of Nω -methyl- l -arginine to generate an N -nitrosourea...

2024年10月25日 · Each sample was cultured in nutritive medium A (NMA) and MRS broth at 37 °C for 24 h under anaerobic conditions until reaching an optical density at 600 nm (OD 600) of 0.5–0.6, which was measured every 2 h with an HP/Agilent 8453 UV–VIS spectrophotometer (SpectraLab Scientific, USA) to isolate raw bacterial consortia (BC) .

2020年6月8日 · Taken together, this study characterized NMA production by metabolically engineered C. glutamicum, and a first bioreactor process leading to a final titer of 0.5 g·L −1 NMA with a volumetric productivity of 0.01 g·L −1 ·h −1 and a yield of 4.8 mg·g −1 glucose was achieved. This strain provides the basis to develop an industrially ...

2014年10月19日 · Colonies of all Listeria species growing on media containing agar show limited visual variation and after 24–48 h are 0.5–1.5 mm in diameter, round, translucent, low convex with a smooth surface and entire margin, and nonpigmented with a crystalline semitransparent central appearance. Growth may be sticky when removed from agar surfaces but ...

2023年10月19日 · 亚利桑那菌琼脂(SA) Salmonella Arizona Agar. HE 琼脂(HE) Hekton Enteric Agar. 三糖铁琼脂(TSI) Triple Sugar Iron Agar. 马铃薯葡萄糖琼脂 (PDA) Potato Dextrose Agar. 高盐察氏琼脂 Salt Czapek Dox Agar. 沙氏琼脂培养基 Sabouraud’s Agar. 孟加拉红培养基 Rose Bengal Medium. YPD琼脂Yeast Peptone Dextrose Agar