Analysis of T cells in mouse lymphoid tissue and blood with flow ...
2021年3月19日 · This step details the staining of splenic, thymic and blood single-cell suspensions with fluorophore-conjugated anti-mouse antibodies (see Tables 1, 2, 3, 4, 5, 6, 7, and 8) to study T cells in spleen, thymus, and blood. Single-stained cell control samples are needed to set the PMT voltage for each parameter and single-stained eBeads (UltraComp ...
Optimized immunofluorescence staining protocol for imaging …
2021年9月17日 · Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues.
Cut each frozen section at 6μm and mount on a positively charged slide. Immediately fix the section in Rapid Fix Solution for 7 seconds. Rinse the slide thoroughly in tap water to remove excess fixative and then place in 1X Wash Buffer. Once all the slides have undergone this process, proceed to step 2.
Multispectral Imaging of T and B Cells in Murine Spleen and Tumor
2016年5月1日 · Interestingly, we observed the presence of a small population of cells that stained with CD8α in Rag1 −/− mice; however, these CD8α + cells did not stain with CD3 (Fig. 2A, 2E). Based on their morphology and location within the spleen, they are most likely CD3 − CD8α + dendritic cells (DCs) .
Isolation and enrichment of mouse splenic T cells for ex vivo …
Herein, we describe protocols for TCR stimulation assays, including procedures for the isolation and enrichment of mouse splenic T cells for ex vivo TCR stimulation with anti-CD3/CD28 antibodies, and the use of ovalbumin-OT-II mouse model for in vivo TCR stimulation.
Immunohistochemical staining for CD3 in (A) the thymus and (B) …
Immunohistochemical staining for CD3 in (A) the thymus and (B) the spleen. The T cell zone in each tissue was specifically stained for CD3 in human CD3 EDG–replaced mice and in wild-type...
Flow cytometric analysis of normal and reactive spleen
2004年4月23日 · Splenocytes were stained with the following antibody combinations: CD20 FITC/Kappa PE/CD45 PerCP/CD19 APC (APC: allophycocyanine), CD20 FITC/ Lambda PE/CD45 PerCP/CD19 or isotype matched control...
Immunophenotyping | mouse T cells from spleen - Miltenyi Biotec
Gating strategy showing the analysis of T cell subsets from mouse spleen. Splenocytes from C57BL/6 mice were stained using the previously described T cell panel to identify major T cell subsets. CD4 + and CD8 + T cells were identified from CD3-expressing cell subsets (A, B).
A novel Ly6C/Ly6G‐based strategy to analyze the mouse splenic myeloid ...
2011年12月29日 · Currently, there is no standardized panel for immunophenotyping myeloid cells in mouse spleen using flow cytometry. Markers such as CD11b, CD11c, F4/80, Gr-1, Ly6C, and Ly6G have long been used to identify various splenic cell myeloid populations.
Characterization of immune cell subtypes in three commonly used mouse ...
2018年10月23日 · In order to determine the distribution of individual immune cell subtypes in BM and splenic isolates of three commonly used mouse strains, we conducted an unbiased analysis of cells from BM and...