We employ advanced molecular techniques, specifically polymerase chain reaction (PCR) testing, for identifying microbes in various specimen types. Rapid microbe identification is determined by PCR and then combined with our molecular phenotypic antibiotic susceptibility testing platform.

Concept：聚合酶链式反应（polymerase chain reaction，PCR）模拟体内DNA复制条件，应用DNA聚合酶反应，体外程序化地特异性扩增某一DNA片段的技术。 PCR的最大特点，是能将微量的DNA大幅增加

Next-generation sequencing (NGS) and polymerase chain reaction (PCR) are culture-independent molecular methods available for commercial use to diagnose UTI. To systematically evaluate the evidence comparing the diagnostic and therapeutic values of molecular diagnostic methods to urine culture in the management of UTI in adults.

Mokan Labs, LLC’s Post Mokan Labs, LLC 262 followers 5d Report this post The future of Kidney Stone testing # ... PCR for Kidney Stone Pathogen Detection: The Future of Urology

Our testing platform provides the benefits of rapid molecular pathogen identification and adds true, phenotypic antibiotic susceptibility - providing faster, more actionable results for patients and healthcare providers. Learn more about our Molecular …

2021年4月25日 · rt-pcr原理是：提取组织或细胞中的总rna，以其中的mrna作为模板，采用oligo(dt)或随机引物利用逆转录酶反转录称cdna。再以cdna为模板进行pcr扩增，而获得目的基因的表达。rt-pcr使rna检测的灵敏性提高了几个数量级，使一些极为微量rna样品分析成为可能。

In multiplex polymerase chain reaction (PCR) meta-analysis, overall sensitivity was 0.80 (95% confidence interval [CI] 0.73-0.86) and specificity was 0.83 (95% CI 0.52-0.95). For the DOR, the overall result was 21 (95% CI 4.8-95).

聚合酶連鎖反應 （英語： Polymerase chain reaction， 縮寫： PCR）又稱 多聚酶链式反應，是一项利用 DNA双链复制 的原理，在生物体外复制特定DNA片段的核酸合成技术。 透过这項技术，可在短时间内大量扩增 目的基因 （標的基因） [1][2]，而不必依賴 大腸桿菌 或 酵母菌 等生物體。 DNA 体外扩增的实现首先基于DNA半保留复制原理，还有碱基互补配对原则，即复制的过程中双链DNA会解链，由双链变成单链，温度一般为95℃；之后温度降下来，引物会结合到DNA单 …

甲基化特异PCR （MSP，methylation-specific PCR）的基本原理是 重亚硫酸盐 （或者亚硫酸氢盐）使DNA中未发生甲基化的胞嘧啶脱氨基转变成尿嘧啶，而甲基化的胞嘧啶保持不变，用PCR扩增所需片段，则尿嘧啶全部转化成胸腺嘧啶，最后，对PCR产物进行测序并且与未经 ...

2023年3月6日 · The polymerase chain reaction (PCR) is a laboratory nucleic acid amplification technique used to denature and renature short segments of DNA using DNA polymerase I enzyme, an isolate from Thermus aquaticus, known as Taq polymerase. [1] [2] In 1985, PCR was introduced by Mullis et al, who were later awarded the Nobel Prize for their work. [3] .