MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.

MitoSOX is among the most commonly used probe for detecting cellular ROS. MitoSOX accumulates in mitochondria due to its positive charge, and MitoSOX-based assays, especially flow cytometry, have been frequently used to detect mitochondrial ROS formation in cells under various conditions.

2022年7月15日 · MitoSOX Red is a live cell fluorescent probe that specifically targets mitochondria and is cell membrane permeable. MitoSOX Red enters mitochondria and is oxidized by superoxide but not by other ROS or RNS generating systems.

MitoSOXTM Red mitochondrial superoxide indicator is a novel fluorogenic dye for highly selective detection of super-oxide (Figure 1) in the mitochondria of live cells. MitoSOXTM Red reagent is live-cell permeant and is rapidly and selec-tively targeted to the mitochondria.

New MitoSOX Green Mitochondrial Superoxide Detection Reagent. Visualize mitochondrial superoxide in your live cells with specificity and ease-of-use, MitoSOX Green offers: Highly selective for mitochondrial superoxide, not other ROS/NOS; Easy imaging with a standard FITC/GFP fluorescence filter on any fluorescence microscope

Many of the applications of MitoSOX™ Red indicator for specific detection of mitochondrial superoxide in live cells (Figure 1) and tissues discussed in this article revolve around SOD and Complex I, disorders in their expression, structure, and function, and their ultimate consequences in pathologies including amyotrophic lateral sclerosis ...

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of …

MitoSOX (Mitochondrial Superoxide Indicators) are fluorescent probes specifically designed to detect and quantify mitochondrial superoxide (O2-) (Dikalov & Harrison, 2014).

We describe use dyhydroethidium (DHE) and MitoSOX (a derivative of dihydroethidium bound to a lipophilic cation) to detect mitochondrial superoxide in yeast and mammalian cells, respectively. We also describe use of genetically encoded fluorescent biosensors for quantitative analysis of mitochondrial NADPH levels (iNap) in mammalian cells and ...

Isolated T-cells are incubated with test-nanoparticles, and the generation of the ROS is detected using MitoSOX reagent. MitoSOX reagent is a cell-permeable fluorogenic probe which is non-fluorescent when in resting state but emits a strong fluorescent signal in …