Background: By simultaneously staining multiple immunomarkers on a single tissue section, multiplexed immunohistochemistry (mIHC) enhances the amount of information that can be observed in a single tissue section and thus can be a powerful tool to visualise cellular interactions directly in the tumour microenvironment.

The multiplex immunohistochemistry (mIHC) platform provides an automated, tissue sparing and cost- effective solution for multiplex analysis of up 23 immune markers on a single FFPE slide. mIHC analysis allows for thorough investigation of in situ immune contexture of tumor and stroma.

Multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) technologies, which allow the simultaneous detection of multiple markers on a single tissue section, have been introduced and adopted in both research and clinical settings in response to …

Multiplex immunohistochemistry (mIHC) enables simultaneous staining of multiple immune markers on a single tissue section. Mounting studies have demonstrated the versatility of mIHC in evaluating immune infiltrates in different diseases and the tumour microenvironment (TME).

Conclusions: mIHC/IF represents a promising tool in the era of cancer immunotherapy, as it can simultaneously detect and quantify PD-L1 labelling with multiple antibody clones, and allow accurate evaluation of tumour and immune cells. Clinicians and pathologists require this information to predict patient response to anti-PD-1/PD-L1 therapy.

2017年11月14日 · We present an mIHC platform combining fluorescent and chromogenic staining with automated whole-slide imaging and integrated whole-slide image analysis, enabling simultaneous detection of six...

Chromogenic-Based mIHC Is Widely Based on Immunohistochemical (Standard Single-Antibody Chromogenic Immunohistochemistry) Technologies. One of the most common diagnostic methods is the standard IHC. The IHC technique detects cell proteins using specific antibodies as a result of the antigen–antibody reaction.

2018年7月24日 · Multiplex IHC data quantitated immune subset number present intra-tumoral (IT) vs the tumor stroma, plus distance of immune subsets from the tumor margin (TM). In addition, mIHC showed a close...

Highly multiplexed immunohistochemistry (mIHC) enables the staining and quantification of dozens of antigens in a tissue section with single-cell resolution. However, annotating cell populations that differ little in the profiled antigens or for which the antibody panel does not include specific markers is challenging.

2025年3月31日 · Multiplex IHC - Being able to visualize multiple targets simultaneously is important in research fields like immunology & oncology. Learn more here.