De-multiplexing, adapter trimming and read overlapping for NGS data. Extraction of UMI sequences. © Copyright 2015, Mikhail Shugay. Revision 57d27950. Built with Sphinx using a …

Here we provide a modified protocol for performing nearly error-free high-throughput sequencing of TCR and antibody repertoires, as well as a novel error-correction algorithm that successfully …

All routines in the pipeline are available in manual and batch variants. Batch variants are designed to automatically handle several input samples with minimal shell scripting glue between …

这里我们选择MiXCR进行讲解，因为这个示例文章也是这个MiXCR流程。而且MiXCR是MiLaboratory开发的，他们实验室出品了3款：MiXCR, MIGEC, VDJtools ，都是值得推荐的 …

2014年5月4日 · Here we report MIGEC (molecular identifier groups–based error correction), a strategy for high-throughput sequencing data analysis. MIGEC allows for nearly absolute error …

MIGEC can be used as pre-processing tool to assemble full-length consensuses for further post-analysis withHIgBlast tool. Note that due to typically poor quality of MiSEQ 300+300bp reads, …

A RepSeq processing swiss-knife. Contribute to mikessh/migec development by creating an account on GitHub.

The purpose of this release is to store binaries so that MIGEC can be installed with Homebrew package manager. Add a shell script that simplifies invocation of MIGEC routines; Fixed a bug …

To install it get the latest JRE and download the executable from releases section. To ran a specific script from the pipeline, say Checkout, execute. Where $VERSION stands for pipeline …

2015年2月18日 · One of the main applications of immune repertoire sequencing is to identify the sequences of CDR3 regions of immune genes (T-cell receptors and immunoglobulins) which …