GTTs and ITTs in mice: simple tests, complex answers
2021年6月11日 · The two most common tests for determining metabolic health in mice are the glucose tolerance test (GTT) and insulin tolerance test (ITT).
一文掌握「小鼠葡萄糖代谢」相关试验要点 - 搜狐
2020年11月8日 · 肥胖是造成代谢紊乱如「胰岛素抵抗」的常见危险因素，对于肥胖的研究，最常见的动物模型就是 60% 高脂饮食诱导 C57BL/6J 小鼠肥胖，此模型与临床肥胖症的病理相似，稳定性好，10 周左右就可以看到明显的表型。 表型出现后，除了测量体重之外，更需要衡量的就是体内胰岛素抵抗水平和葡萄糖耐量，通俗来说就是机体对葡萄糖分解代谢的能力，这个时候我们可以外源性地给小鼠葡萄糖后来监测一定时间段内的血糖情况，也就是葡萄糖耐量检测，
The Glucose Tolerance Test in Mice - PubMed
The GTT measures changes in blood glucose concentration over a 2-h period following the administration of a bolus of glucose. However, this simplicity belies several important considerations which need to be addressed, to aid reproducibility and produce interpretable data.
Guidelines and Considerations for Metabolic Tolerance Tests in Mice
In mice, the GTT is the simplest, and usually the first, test applied to an animal model, and it provides a physiological overview of any changes in glucose tolerance without determining the causative mechanisms. Briefly, mice are routinely fasted before the administration of glucose.
Revisited guidelines for metabolic tolerance tests in mice
2024年11月25日 · Glucose tolerance tests (GTT) and insulin tolerance tests (ITT) are by far the most common and simple metabolic tests used by researchers to assess glucose...
The glucose tolerance test in mice: Sex, drugs and protocol
To establish the impact of sex, dosing route, fasting duration and acute habituation stress on glucose tolerance test (GTT) measurements used in the preclinical evaluation of potential glucose‐modulating therapeutics. Adult male and female C57Bl/6J ...
The Glucose Tolerance Test in Mice - Springer
2020年3月17日 · The GTT is a simple test which can be used to generate fast accurate metabolic data regarding glucose tolerance. Its interpretation is most powerful when performed in conjunction with the measurement of plasma insulin during the GTT and with an understanding of insulin sensitivity derived either from an insulin tolerance test or clamp studies.
In an insulin tolerance test, insulin is injected, and the change in blood glucose is followed, as with a GTT. IP injection is the most common delivery route for insulin, although intravenous...
Glucose and Insulin Tolerance Tests in the Mouse
The glucose tolerance test (GTT) measures the clearance by the body of an injected glucose load [9]. A solution of glucose is administered by intraperitoneal injection in overnight-fasted mice, and subsequently plasmatic glucose levels are measured at …
GTTs and ITTs in mice: simple tests, complex answers
The article discusses the complexity of glucose and insulin tolerance tests in mice.