2019年3月6日 · To study DNA-induced autophagy, we transfected interferon-stimulatory DNA (ISD) into BJ cells, which are immortalized human fibroblasts. DNA transfection not only …

2017年12月12日 · We first transfected mouse embryonic fibroblast (MEF) with HT-DNA (herring testes DNA) (a commonly used double-strand DNA [dsDNA] ligand for cGAS) to activate the …

2018年10月22日 · Human osteosarcoma U2OS cells expressing GFP-LC3 were incubated with Redp, at the indicated concentrations, for 20 h followed by addition of BFA (5 µg/mL) or GCA …

小鼠胚胎成纤维细胞 (MEF)原代培养可应用于：（1）细胞保种；（2）分子生物学研究；（3）基因治疗相关研究。 操作方法. 胰酶消化法1. 原理. 将小鼠的胚胎成纤维细胞（MEF）从机体中 …

2018年12月19日 · STING (stimulator of interferon genes) is a central molecule that binds to cyclic dinucleotides produced by the cyclic GMP-AMP synthase (cGAS) to activate innate immunity …

为了研究溶酶体损伤和 EV 分泌之间的关系，作者用液泡 H+ ATP 酶 (V-ATP 酶) 抑制剂巴弗洛霉素 A1 (Baf A1) 处理小鼠胚胎成纤维细胞 (MEF)，并检查这些细胞的 EV 释放情况。 使用差速离心 …

2016年7月20日 · Furthermore, treatment with BFA inhibits autophagy in the ATG5 − cells but not in the ATG5 + cells. Similar results are obtained in ATG7 − MEFs. The noncanonical BECN1 …

2013年5月28日 · We previously showed that the fungal toxin brefeldin A (BFA) induces the ADP-ribosylation of C-terminal–binding protein-1 short-form/BFA–ADP-ribosylation substrate …

2009年12月1日 · To develop a feeder-free culture system for optimizing the propagation of mouse iPS cells, we compared the abilities of bFGF plus activin A (bFA) and MEF-CM to maintain the …

2020年3月6日 · BFA inhibited both Gln- and Asn-induced mTORC1 activation in both WT and Rag A/B KO MEFs (Fig. 4, A–C). Moreover, BFA treatment in WT MEF cells dramatically …