Historically, MeCP2 has been characterized as a proximal gene silencer with 2 functional domains: a methyl DNA binding domain and a transcription repression domain. However, several lines of new data indicate that MeCP2 structure and function relationships are more complex.

MeCP2 is a nuclear protein characterized by several functionally annotated domains (Figure 1). These domains include a MBD and a NCoR-interacting domain (NID) that regulate the binding of MeCP2 to methylated DNA and the consequent transcriptional repression [5].

The MECP2 gene is located on the long (q) arm of the X chromosome in band 28 ("Xq28"), from base pair 152,808,110 to base pair 152,878,611. MECP2 is an important reader of DNA methylation. Its methyl-CpG-binding (MBD) domain recognizes and binds 5-mC regions. MECP2 is X-linked and subject to X inactivation.

2024年12月25日 · MECP2 (Methyl-CpG Binding Protein 2) is a Protein Coding gene. Diseases associated with MECP2 include Rett Syndrome and Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations. Among its related pathways are Transcriptional Regulation by MECP2 and Gene expression (Transcription).

The recent delineation of at least six biochemically distinct domains within MeCP2 , along with the recognition that MeCP2 has a disordered tertiary structure potentially allowing different combinations of domains to act together for specific functions , provide a strengthened framework for understanding the various structural elements within ...

Structural studies using chromatin assembled in vitro have revealed that MeCP2 can associate with nucleosomes in an N-terminus dependent manner and efficiently condense nucleosome arrays. The present review attempts to match MeCP2 structural domains, or lack thereof, and specific chromatin features needed for the proper recruitment of MeCP2 to ...

2024年8月20日 · We discovered that MeCP2 exhibits distinct one-dimensional diffusion kinetics when bound to unmethylated versus CpG methylated DNA, enabling methylation-specific activities such as co-repressor...

2022年6月1日 · Describes basic epigenetic mechanisms including eukaryotic transcriptional regulation. Describes the distinct topologically associating domains of the chromatin structure. Explains experimental methods to study newly synthesized transcripts. Discusses the role of an epigenetic reader “MeCP2″ in controlling transcriptional gene regulation.

2025年3月26日 · Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters.

2021年6月25日 · MeCP2 consists of several domains, including the methyl-CpG-binding domain (MBD), the transcriptional repression domain (TRD), the NCoR-SMRT interaction domain (NID), and the AT-hook motifs (AT) . MBD and NID are both important for MeCP2 function (10–12).