MCF 10A was derived from adherent cells in the population. The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures.

The MCF10A human mammary epithelial cell line is a widely used in vitro model for studying normal breast cell function and transformation. However, there is limited knowledge about whether MCF10A cells reliably represent normal human mammary cells.

MCF10A is a human mammary epithelial cell line. It is a “normal” cell line that was spontaneously immortalized. Unlike cancer lines, MCF10A grows in medium supplemented with additional growth factors etc. The attachment of the cell to TC dish is tight.

MCF10A cells are instrumental in vitro for elucidating the intricacies of normal mammary epithelial cell functions, encompassing cell-cell adhesion mediated by proteins like E-cadherin, morphogenetic processes, and intricate signaling cascades.

2021年8月15日 · Because of the establishment of MCF10, the use of its most well-known parental line, MCF10A, has allowed breast cancer researchers to model key aspects of normal and aberrant breast epithelial biology.

MCF10A is a spontaneously immortalized, non-malignant breast cell line obtained from a patient with benign fibrocystic disease [32] and is the founder cell line of a progressively more aggressive family of breast cancer lines.

Immortal human mammary epithelial cell lines. Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.

2022年10月7日 · Comprehensive profiling of ligand-induced perturbation responses of the MCF10A mammary epithelial cell line provides an exhaustive resource for identification of the molecular basis of cellular...

Here, we created an ECAD-EmGFP reporter cell line (ATCC CRL-10317EMT) using the CRISPR/Cas9 gene editing platform and the parental MCF 10A (ATCC CRL-10317). The created CRL-10317EMT cell line harbors a C-terminal green …

2024年1月4日 · To add to the knowledge of the effects of TP53 and PTEN mutations on the DDR network, we performed RNA-seq and phosphoproteomic profiling of three congenic cell lines (MCF10A, MCF10A...