The MCF 10A ECAD-EmGFP reporter cell line provides a convenient and sensitive platform for research on the mechanisms of metastasis in vitro and the development of new antitumor drugs for metastatic breast cancer.

Immortal human mammary epithelial cell lines. Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.

MCF 10A cell lines are immortalized, non-tumorigenic epithelial cells derived from human breast tissue. They are extensively used as in vitro models to study breast tumor progression due to their close mimicry of normal breast epithelium and their ability to undergo oncogenic transformation.

The human normal breast cell line MCF-10A is being widely used as a model in toxicity studies due to its structural similarity to the normal human mammary epithelium. Over the years, application of carbon dots (C-dots) in biomedicine has been ...

MCF-10A cells are commonly recognized as a ‘‘normal’’ breast epithelial cell line, the karyotype is stable and near-diploid, these cells are nonetheless cytogenetically abnormal. These cells harbor a deletion of the locus containing p16 and p14ARF, as well as amplification of MYC. Notably, MCF-10A cells express wild-type p53.

2015年7月6日 · MCF10A cells exhibit a unique differentiated phenotype in 3D culture which may not exist or be rare in normal human breast tissue. Our results raise a question as to whether the commonly used MCF10A cell line is a suitable model for human mammary cell studies.

2025年3月14日 · Explore the biological complexity of MCF 10A cells, from growth behavior to epigenetic regulation and 3D culture applications in research. MCF 10A cells are a widely used, non-tumorigenic breast epithelial cell line that serves as a valuable model in cancer research and cellular biology.

MCF-10A, immortalized but non-transformed human breast epithelial cells, are widely used in research examining carcinogenesis. The studies presented here were initiated with the observation that MCF-10A cells left in continuous culture for prolonged ...

Here we provide a collection of protocols to culture MCF-10A cells, to establish stable pools expressing a gene of interest via retroviral infection, as well as to grow and analyze MCF-10A cells in three-dimensional basement membrane culture.

2020年12月10日 · In this work we prepared three different C-dots from the same set of carbon and nitrogen precursors (citric acid and urea, respectively) via three distinct bottom-up synthetic routes and their safety was tested against the normal breast cell line MCF-10A.