2022年6月23日 · Ultimately, we successfully deliver several variants that separately exhibit both enhanced preference and catalytic efficiency to three widespread C6 sugar-phosphates, namely, glucose 6-phosphate...

2019年5月7日 · PMI是一种锌依赖性酶，可催化甘露糖6磷酸（M6P）和果糖6磷酸（F6P）之间的可逆异构化，可能是通过形成顺式烯二醇中间体。 假设该反应涉及M6P的开环，质子从C2原子转移到C1原子以及在底物的O1和O2原子之间转移，然后闭环产生产物F6P。 已经进行了一些尝试来解释锌离子和各种残基在PMI催化功能中的作用。 但是，关于催化碱和酶催化反应的机理尚无共识。 在本研究中，基于鼠伤寒沙门氏菌的PMI结构，针对靠近结合金属离子的残基的定点诱变和 …

2024年6月1日 · In ΔpfkA strain, a recombinant synthetic pathway based on mannose-6-phosphate isomerase and YniC was developed to direct F6P to mannose. The resulting strain successfully produced 25.2 g/L mannose from glucose with a high conversion rate of 63% after transformation for 48 h.

2024年12月19日 · 然而，溶酶体靶向蛋白并没有特定的靶向序列，而是通过一种糖基化修饰，即甘露糖-6-磷酸（M6P）标签实现。 溶酶体功能： 含有多种酸性水解酶，参与降解细胞内外的蛋白质、多糖、脂质和核酸等。 酸性水解酶的错误定位会导致细胞外环境破坏或代谢紊乱。 M6P标签的形成机制. 蛋白质的初步合成与糖基化： 溶酶体酶首先在核糖体合成并转运到内质网（ER）。 N-连接糖基化：甘露糖残基附加到蛋白质上。 甘露糖-6-磷酸（M6P）标签的添加： 位置： 高尔 …

2003年3月1日 · ;Mannose 6-phosphate (M6P) receptors (the CD-MPR and CI-MPR) are type-I integral membrane glycoproteins that constitute the family of p-type lectins. They bind to M6P-containing lysosomal...

Mannose-6-phosphate (M6P) is a molecule bound by lectin in the immune system. M6P is converted to fructose 6-phosphate by mannose phosphate isomerase. M6P is a key targeting signal for acid hydrolase precursor proteins that are destined for transport to lysosomes. The M6P tag is added to such proteins in the cis - Golgi apparatus.

The cation-independent mannose 6-phosphate receptor is a multifunctional protein which binds at the cell surface to two distinct classes of ligands, the mannose 6-phosphate (M6P) bearing proteins and IGF-II.

2012年4月1日 · The explanation of how are the lysosomal enzymes accurately recognized and selected over many other proteins in the trans -Golgi network relies on being tagged with a unique marker: the mannose-6-phosphate (M6P) group, which is added exclusively to the N-linked oligosaccharides of lysosomal soluble hydrolases, as they pass through the cis -Golgi...

In this study, we found that probiotics may reduce the production of NO and reduce intestinal inflammation by downregulating the NOS2 gene; downregulation of GAD1 gene reduces GABA production and...

The explanation of how are the lysosomal enzymes accurately recognized and selected over many other proteins in the trans-Golgi network relies on being tagged with a unique marker: the mannose-6-phosphate (M6P) group, which is added exclusively to the N-linked oligosaccharides of lysosomal soluble hydrolases, as they pass through the cis-Golgi n...