Lipo2000 usually works Ok for me, not much cell death. However, of the last 4-5 years or so, I mostly use Effectene from QIAGEN, high efficiency, low toxicity, need less DNA, no need to …

Usually I use Lipo2000 for plasmids, but Lipo2000 works well with shRNA, too.-Andrea Fortina-

2008年1月10日 · Help: MIN6 Transfection 96well plate Lipo2000 - (Jan/08/2008 ) Pages: 1 2 Next Hi, I'm wondering if anyone has successfully transfected MIN6 (P25) in 96 well plate using …

I have done 12 kb plasmid with lipo2000; your 18 kb plasmid is not much larger. I know ppl use adenoviral plasmid (~30 kb) to make recombinant virus. So the size of 18 kb should not be the …

2007年7月23日 · All transfection reagents are more or less toxic. Hep G2 seems to be more resistant than other cell lines that I have worked with. I dont recall any serious problem with …

Lipo2000 is used to transfect package cell (pheonix-Eco, which is 293 modified) Day 1 I transfected plasmid to package cells in the afternoon Day 2 I change medium in the morning …

I am currently doing a luciferase assay with pGL3 vector (400ng) and co-transfecting a mature miRNA of interest and a cel-miR negative control (both mimics are from Dharmacon). I use …

2007年9月6日 · Lipo2000 is pretty expensive. It is better than LPEI.-genehunter-1-QUOTE (genehunter-1 @ Sep 6 2007, 11:21 ...

Lipo2000 is a general transfection reagent that works on many different types of cells. Is it suspension cell type? if so it will be more difficult to transfect. Let us know your finding and …

This should be a good enought time period for the liposomes to have entered the cells. I usually let the transfection go for 5-7 hours before changing the media using Lipo2000 as well.