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HEK 293 cells - Wikipedia
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. [1] [2] The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection.
hek293t和293t细胞的区别是什么? - 知乎专栏
2023年2月20日 · HEK293 是一种永生化细胞系,来源于人胚胎肾脏,用剪切的人 腺病毒 5 型 DNA 转染以产生许多重组蛋白。 HEK293 刚开始是由荷兰生物学家 Alex Van der Eb 于 1973 年从人类肾脏细胞中分离出来的。 这些细胞是在女性胎儿的组织培养物中生长的。 后来,Van der Eb 实验室的博士后研究员 Frank Graham 对它们进行了剪切的人类腺病毒 5 型 DNA 转染。 该细胞系之所以被命名为HEK293,是因为这是弗兰克的第293次实验。 将腺病毒基因整合到 HEK 细 …
干货 | 十个让生活变得更美好的lifehack - 知乎 - 知乎专栏
在英语里,这一类生活妙招都可以用 lifehack 来表示。 Lifehack refers to any trick, shortcut, skill, or novelty method that increases productivity and efficiency in all walks of life.
200 Incredible Life Hacks That Make Life So Much Easier
2022年2月18日 · We have collected 200 most practical life hacks into this one big list. By tweaking little things in your daily life, it can make your life much easier.
HEK | Primary Human Epidermal Keratinocytes - Cell Applications
Human Epidermal Keratinocytes (HEK) provide an excellent model to study epithelial function and disease, skin biology, toxicology and HPV infection. When grown on inserts and provided a liquid/air interface, they differentiate into a stratified squamous epithelium, forming a …
100 BEST LIFE HACKS OF ALL TIME
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角质细胞-角质形成细胞培养基-赛默飞 | Thermo Fisher Scientific - CN
正常新生儿角质形成细胞 (HEkn) (C-001-5C) 在 EpiLife 培养基和主要竞争对手的角质形成细胞培养基中的生长情况。 与在竞争对手培养基中生长的细胞相比,EpiLife 培养基中生长的培养物可在更长的时间内显示出群体倍增。 如果您想培养细胞…… 当最大程度减少实验室中的浪费时、一点一滴都有帮助。 1升的Gibco培养基瓶比现在市场上的同类培养基瓶使用的原料节省38% (0.5升的瓶子节省32%)。 这减少了不可再生资源的消耗。 了解有关可持续解决方案的更多信息. 查找有 …
人表皮角质形成细胞(HEK)培养方案 - MilliporeSigma
在眼睛和手都有适当保护的情况下,从液氮储罐取出冷冻保存的HEK小管。 将瓶盖转动四分之一圈,以释放可能被困在螺纹中的液氮,然后重新拧紧瓶盖。 将小管的下半部分放在37°C水浴中快速解冻细胞,并在解冻过程中密切观察小管。 当小管中仅有少量冰时,从水浴中取出小管。 请勿让细胞完全解冻。 在无菌生物安全柜中用70%酒精对小管外部消毒。 小心取下小管盖。 请勿用手触摸盖子或小管的边缘,以免污染。 用2 mL移液管轻轻吹5次,将细胞重悬于小管中。 小心不 …
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